|Tested species reactivity||Bovine, Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the alpha1 catalytic subunit within exon 8 of human AMP activated protein Kinase (AMPK).|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2-8 µg/mg of lysate|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21909 detects AMPK alpha 1 in bovine, human and rat samples.
PA1-21909 has been successfully used in Immunoprecipitation and Western Blot procedures. Predicted molecular weight: 63 kDa.
The PA1-21909 immunogen is a synthetic peptide corresponding to the alpha1 catalytic subunit within exon 8 of human AMP activated protein Kinase (AMPK).
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.