Immunofluorescent analysis of AMPK alpha 2 in HeLa (left) and A431 (right) cells fixed in 4% paraformaldehyde at RT for 15 min, using an aMPK alpha 2 polyclonal antibody (Product # PA5-21494). Green: Primary antibody at a dilution of 1:400. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21494 targets AMPK alpha 2 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-21494 immunogen is a recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.