Immunohistochemistry analysis of ANGPTL4 showing staining in the cytoplasm and nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- ANGPTL4 Polyclonal Antibody (409800) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Virus, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the human ANGTPL4 protein, which differs from rat and mouse by one and two conservative amino acid replacements, respectively|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Angiopoietin-like protein 4 (ANGPTL4), is a secreted protein selectively expressed in adipose tissue, liver, and placenta1-3 that plays a variety of roles in vivo, ranging from adipogenesis to angiogenesis to carcinogenesis. The expression of ANGPTL4 is also under nutritional and hormonal control. ANGPTL4 has been observed to induce a strong pro-angiogenic response independent of vascular endothelial growth factor (VEGF), and its expression has been described in hypoxic human tissues as well as a variety of cancers, including liposarcoma, hepatocellular carcinoma, and conventional renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression.
40-9800 was used in immunohistochemistry and western blot elucidate how hematopoietic ANGPTL4 deficiency increases atherogenesis
|Aryal B,Rotllan N,Araldi E,Ramírez CM,He S,Chousterman BG,Fenn AM,Wanschel A,Madrigal-Matute J,Warrier N,Martín-Ventura JL,Swirski FK,Suárez Y,Fernández-Hernando C||Nature communications (7:null)||2016|
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
40-9800 was used in western blot to determine the mechanisms of hypertriglyceridemia in Pcyt2 heterozygous mice.
|Singh RK,Fullerton MD,Vine D,Bakovic M||Journal of lipid research (53:1811)||2012|
Angiopoietin like protein 4 expression is decreased in activated macrophages.
40-9800 was used in western blot to examine the effect of TLR activation on the expression of macrophage ANGPTL4.
|Feingold KR,Shigenaga JK,Cross AS,Moser A,Grunfeld C||Biochemical and biophysical research communications (421:612)||2012|
|Not Applicable||3-4 µg/ml||
The acute phase response stimulates the expression of angiopoietin like protein 4.
40-9800 was used in western blot to investigate factors that regulate ANGPTL3 expression
|Lu B,Moser A,Shigenaga JK,Grunfeld C,Feingold KR||Biochemical and biophysical research communications (391:1737)||2010|
|Human||Not Cited||Activating peroxisome proliferator-activated receptor gamma mutant promotes tumor growth in vivo by enhancing angiogenesis.||Tian L,Zhou J,Casimiro MC,Liang B,Ojeifo JO,Wang M,Hyslop T,Wang C,Pestell RG||Cancer research (69:9236)||2009|
|Enhanced angiogenesis in obesity and in response to PPARgamma activators through adipocyte VEGF and ANGPTL4 production.||Gealekman O,Burkart A,Chouinard M,Nicoloro SM,Straubhaar J,Corvera S||American journal of physiology. Endocrinology and metabolism (295:E1056)||2008|
|Human||Not Cited||Peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) ligands do not potentiate growth of human cancer cell lines.||Hollingshead HE,Killins RL,Borland MG,Girroir EE,Billin AN,Willson TM,Sharma AK,Amin S,Gonzalez FJ,Peters JM||Carcinogenesis (28:2641)||2007|
Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (ANGPTL4).
40-9800 was used in western blot to explore the mechanism by which Lactobacillus paracasei ssp paracasei F19 alters fat storage
|Aronsson L,Huang Y,Parini P,Korach-André M,Håkansson J,Gustafsson JÅ,Pettersson S,Arulampalam V,Rafter J||PloS one (5:null)||2010|