Invitrogen

ANKRD2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (5) ANKRD2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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ANKRD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of ANKRD2 in human cell line U-2 OS shows positivity in vesicles. Samples were probed using an ANKRD2 Polyclonal Antibody (Product # PA5-59339).

ANKRD2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ANKRD2 in human skeletal muscle and prostate tissues using ANKRD2 Polyclonal Antibody (Product # PA5-59339). Corresponding ANKRD2 RNA-seq data are presented for the same tissues.

ANKRD2 Antibody in Western Blot (WB) — Western blot analysis of ANKRD2 in control (vector only transfected HEK293T lysate) and ANKRD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ANKRD2 Polyclonal Antibody (Product # PA5-59339).

ANKRD2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ANKRD2 demonstrates antibody specificity. Immunohistochemical analysis of ANKRD2 using anti-ANKRD2 Polyclonal Antibody (Product # PA5-59339), shows significant staining of ANKRD2 in human skeletal muscle and shows minimal or weak staining in human prostate tissues. The relative expression levels of ANKRD2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-59339

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human ANKRD2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2637965

Product Specific Information

Immunogen sequence: IIKLLLLHGA DMMTKNLAGK TPTDLVQLWQ ADTRHALEHP EPGAEHNGLE GPNDSGRETP QPVPAQ

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 88%.

Target Information

ANKRD2 may play an important role in skeletal muscle hypertrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ankyrin repeat domain 2 (stretch responsive muscle); Ankyrin repeat domain-containing protein 2; hArpp; Skeletal muscle ankyrin repeat protein

Gene Aliases: ANKRD2; ARPP

UniProt ID: (Human) Q9GZV1

Entrez Gene ID: (Human) 26287

Function(s)

negative regulation of transcription from RNA polymerase II promoter regulation of cytokine production muscle contraction muscle organ development negative regulation of myotube differentiation skeletal muscle cell differentiation regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of myoblast differentiation regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of myoblast proliferation

Process(es)

chromatin binding structural constituent of muscle titin binding protein kinase B binding RNA polymerase II sequence-specific DNA binding transcription factor binding