Western blot detection of AP1G1 in 20µg of HeLa whole cell lysate (lane 1). Samples were run on a SDS-PAGE, transferred onto nitrocellulose, and probed using an AP1G1 polyclonal antibody (Product # PA1-31775) at a dilution of 1:500 followed by detection using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. Chemiluminescence was used for signal detection at a 60-sec exposure time. Peptide competition (using 1µg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31775 detects AP1G1 from human samples.
PA1-31775 has been successfully used in Western blot applications.
The PA1-31775 immunogen is synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.