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Invitrogen
APAF1 Polyclonal Antibody
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FIGURE: 1 / 2
APAF1 Antibody (PA5-86873) in IHC (P)
Immunohistochemical analysis of APAF1 in paraffin-embedded human tonsil carcinoma tissue showing cytoplasm staining and negative control (right). Sample was incubated with polyclonal APAF1 antibody (Product # PA5-86873) using a 1:50 dilution, and followed by goat anti-rabbit IgG-biotin and avidin-peroxidase secondary antibodies.
Product Details
PA5-86873
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to residues in Human APAF1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-1; Apoptotic protease-activating factor 1; DKFZp781B1145
Gene Aliases: APAF-1; APAF1; CED4; KIAA0413
UniProt ID: (Human) O14727
Entrez Gene ID: (Human) 317
response to hypoxia
neural tube closure
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
nervous system development
aging
response to nutrient
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
glial cell apoptotic process
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
neuron apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to transforming growth factor beta stimulus
response to G1 DNA damage checkpoint signaling
intrinsic apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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