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Invitrogen
APRT Polyclonal Antibody
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FIGURE: 1 / 1
APRT Antibody (PA5-96330) in WB
Western blot analysis of APRT in 293T cells. Samples were incubated with APRT Polyclonal antibody (Product # PA5-96330) using a dilution of 1:2,500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-96330
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_0004761)
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE; Positive Samples: MCF7, 22Rv1, THP-1, A-549, K-562, HeLa, Mouse intestine; Cellular Location: Cytoplasm
Target Information
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; APRT; DKFZp686D13177; MGC125856; MGC125857; MGC129961; transphosphoribosidase
Gene Aliases: AMP; APRT; APRTD; C85684
UniProt ID: (Human) P07741, (Mouse) P08030
Entrez Gene ID: (Human) 353, (Mouse) 11821
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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