A suggested positive control is mouse heart tissue lysate.
PA5-20574 can be used with blocking peptide PEP-0694.
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AC; ACDase; acid CDase; Acid ceramidase; Acid ceramidase subunit alpha; Acid ceramidase subunit beta; Acylsphingosine deacylase; HSD33, AC, ACDase, ASAH, PHP, PHP32; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase; N-acylsphingosine amidohydrolase (acid ceramidase) 1; N-acylsphingosine amidohydrolase 1; PHP32; Putative 32 kDa heart protein
Gene Aliases: 2310081N20Rik; AC; ACDase; ASAH; ASAH1; HSD-33; HSD33; PHP; PHP32; SMAPME