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ASCL1 Antibody (PA5-86633) in WB
Western blot was performed using Anti-ASCL1 Polyclonal Antibody, (Product # PA5-86633) and a ~25 kDa band corresponding to ASCL1 was observed across the panel except in A549, K-562 and HeLa which are reported to be low expressors of ASCL1. An uncharacterized band at ~30 kDa was also observed in U-87 MG cells. SH-SY5Y cells treated with Retinoic acid and PMA also showed a decrease in expression of ASCL1 as compared to the untreated SH-SY5Y cells thus confirming specificity. Modified Whole cell extracts (1% SDS) (30 µg lysate) of SH-SY5Y (La... View More
Product Details
PA5-86633
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 140-190 of Human ASCL1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2803400
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; bHLHa46; Class A basic helix-loop-helix protein 46; KMT2H; mammalian achaete scute homolog 1; mASH-1
Gene Aliases: AI225900; ASCL1; ASH1; BHLHA46; HASH1; Mash-1; MASH1
UniProt ID: (Human) P50553, (Rat) P19359, (Mouse) Q02067
Entrez Gene ID: (Human) 429, (Rat) 64186, (Mouse) 17172
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
bHLH transcription factor binding
E-box binding
double-stranded DNA binding
sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
neuron migration
noradrenergic neuron development
noradrenergic neuron fate commitment
transcription, DNA-templated
Notch signaling pathway
regulation of mitotic cell cycle
neuroblast fate determination
neuroblast proliferation
response to lithium ion
regulation of gene expression
oligodendrocyte development
spinal cord association neuron differentiation
spinal cord oligodendrocyte cell fate specification
vestibular nucleus development
cerebral cortex GABAergic interneuron differentiation
commitment of neuronal cell to specific neuron type in forebrain
central nervous system neuron development
cerebral cortex development
neurogenesis
regulation of epithelial cell differentiation
response to retinoic acid
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of Notch signaling pathway
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell maturation
sympathetic nervous system development
neuron fate specification
musculoskeletal movement, spinal reflex action
response to folic acid
subpallium neuron fate commitment
regulation of timing of subpallium neuron differentiation
olfactory pit development
lung epithelial cell differentiation
ventral spinal cord interneuron fate commitment
lung neuroendocrine cell differentiation
stomach neuroendocrine cell differentiation
carotid body glomus cell differentiation
adrenal chromaffin cell differentiation
sympathetic ganglion development
response to epidermal growth factor
cellular response to magnetism
positive regulation of neural precursor cell proliferation
regulation of transcription from RNA polymerase II promoter
pattern specification process
nervous system development
regulation of Notch signaling pathway
glial cell differentiation
spinal cord oligodendrocyte cell differentiation
oligodendrocyte cell fate commitment
forebrain neuron differentiation
cell differentiation
neuron differentiation
regulation of cell proliferation
neuron fate commitment
neuron development
generation of neurons
oligodendrocyte differentiation
regulation of neurogenesis
positive regulation of neurogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
enteric nervous system development
parasympathetic nervous system development
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