Invitrogen

ASCL1 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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ASCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ASCL1 in paraffin-embedded human brain tissue. Sample was incubated with polyclonal ASCL1 antibody (Product # PA5-86633).

ASCL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ASCL1 Polyclonal Antibody, (Product # PA5-86633) and a ~25 kDa band corresponding to ASCL1 was observed across the panel except in A549, K-562 and HeLa which are reported to be low expressors of ASCL1. An uncharacterized band at ~30 kDa was also observed in U-87 MG cells. SH-SY5Y cells treated with Retinoic acid and PMA also showed a decrease in expression of ASCL1 as compared to the untreated SH-SY5Y cells thus confirming specificity. Modified Whole cell extracts (1% SDS) (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with retinoic acid (1uM for 24hr) (Lane 2), SH-SY5Y treated with PMA (40nM for 48hr) (Lane 3), IMR-32 (Lane 4), U-87 MG (Lane 5), A549 (Lane 6), K-562 (Lane 7) and HeLa (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ASCL1 Antibody in Western Blot (WB) — Western blot analysis of ASCL1 in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: SP2/0 whole cell lysate (40 µg), Lane 4: PC12 whole cell lysate (40 µg), Lane 5: H9C2 whole cell lysate (40 µg). Sample was incubated with ASCL1 polyclonal antibody (Product # PA5-86633) using a 1:500 dilution.

ASCL1 Antibody in Western Blot (WB) — Western blot analysis of ASCL1 in Lane 1: HEK293T cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: H9C2 cell lysate. Sample was incubated with ASCL1 polyclonal antibody (Product # PA5-86633) using a 1:500 dilution.

ASCL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of ASCL1 was observed in SH-SY5Y, IMR-32 and U-87 MG cell lines and not in A549, K-562 and HeLa cell lines using ASCL1 Polyclonal Antibody (Product # PA5-86633) in western blot. {RE}

ASCL1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti- ASCL1 Polyclonal Antibody (Product # PA5-86633) , shows decrease in expression of ASCL1 in SH-SY5Y cell line upon treatment with Retinoic Acid and PMA. {TM}

Product Details

PA5-86633

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide, corresponding to amino acids 140-190 of Human ASCL1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2803400

Target Information

ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; bHLHa46; Class A basic helix-loop-helix protein 46; KMT2H; mammalian achaete scute homolog 1; mASH-1

Gene Aliases: AI225900; ASCL1; ASH1; BHLHA46; HASH1; Mash-1; MASH1

UniProt ID: (Human) P50553, (Rat) P19359, (Mouse) Q02067

Entrez Gene ID: (Human) 429, (Rat) 64186, (Mouse) 17172

Molecular Function: basic helix-loop-helix transcription factor

negative regulation of transcription from RNA polymerase II promoter neuron migration noradrenergic neuron development noradrenergic neuron fate commitment transcription, DNA-templated Notch signaling pathway regulation of mitotic cell cycle neuroblast fate determination neuroblast proliferation response to lithium ion regulation of gene expression oligodendrocyte development spinal cord association neuron differentiation spinal cord oligodendrocyte cell fate specification vestibular nucleus development cerebral cortex GABAergic interneuron differentiation commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development cerebral cortex development neurogenesis regulation of epithelial cell differentiation response to retinoic acid negative regulation of apoptotic process positive regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development neuron fate specification musculoskeletal movement, spinal reflex action response to folic acid subpallium neuron fate commitment regulation of timing of subpallium neuron differentiation olfactory pit development lung epithelial cell differentiation ventral spinal cord interneuron fate commitment lung neuroendocrine cell differentiation stomach neuroendocrine cell differentiation carotid body glomus cell differentiation adrenal chromaffin cell differentiation sympathetic ganglion development response to epidermal growth factor cellular response to magnetism positive regulation of neural precursor cell proliferation regulation of transcription from RNA polymerase II promoter pattern specification process nervous system development regulation of Notch signaling pathway glial cell differentiation spinal cord oligodendrocyte cell differentiation oligodendrocyte cell fate commitment forebrain neuron differentiation cell differentiation neuron differentiation regulation of cell proliferation neuron fate commitment neuron development generation of neurons oligodendrocyte differentiation regulation of neurogenesis positive regulation of neurogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development enteric nervous system development parasympathetic nervous system development

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity bHLH transcription factor binding E-box binding double-stranded DNA binding sequence-specific DNA binding protein dimerization activity