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Invitrogen
ASCL2 Polyclonal Antibody
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FIGURE: 1 / 3
ASCL2 Antibody (PA5-41489) in ICC/IF
Immunofluorescence analysis of ASCL2 was performed using HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ASCL2 Polyclonal Antibody (Product # PA5-41489) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 m... View More
Product Details
PA5-41489
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the C-terminal of human ASCL2 (aa 121-170).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Peptide sequence: RPQAVRPSAP RGPPGTTPVA ASPSRASSSP GRGGSSEPGS PRSAYSSDDS
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 79%; Pig: 100%; Rat: 93%
Target Information
This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: achaete-scute complex homolog 2; achaete-scute complex-like 2; Achaete-scute homolog 2; ASH-2; bHLHa45; Class A basic helix-loop-helix protein 45; H M bHLHa45; mammalian achaete/scute homologue 2; Mash2
Gene Aliases: ASCL2; ASH2; BHLHA45; HASH2; MASH2
UniProt ID: (Human) Q99929
Entrez Gene ID: (Human) 430
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
bHLH transcription factor binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
in utero embryonic development
placenta development
regulation of transcription from RNA polymerase II promoter
nervous system development
sensory organ development
negative regulation of Schwann cell proliferation
stem cell population maintenance
cell differentiation
neuron differentiation
T cell differentiation
somatic stem cell population maintenance
negative regulation of T-helper 1 cell differentiation
negative regulation of T-helper 2 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of neurogenesis
spongiotrophoblast layer development
chorionic trophoblast cell development
T follicular helper cell differentiation
negative regulation of T-helper 17 cell differentiation
positive regulation of T cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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