Search
Alomone Labs, LTD
ASIC1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 4
ASIC1 Antibody (ASC-014-GP200UL) in ICC/IF
Expression of ASIC1 in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized C6 glioma cells. A. Cells were stained using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:200) followed by Anti-guinea pig-AlexaFluor-594 secondary Antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
ASC-014-GP200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Guinea pig
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1, Intracellular, C-terminus
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL
Amount
160 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ACCN2 variant 3; acid sensing (proton gated) ion channel 1; acid-sensing (proton-gated) ion channel 1; acid-sensing ion channel 1a protein; acid-sensing ionic channel; amiloride-sensitive cation channel 2, neuronal; BNaC2; brain sodium channel 2; Cation channel, amiloride-sensitive, neuronal, 2; degenerin 2; hBNaC2; proton gated cation channel ASIC1; unnamed protein product
Gene Aliases: ACCN2; ASIC; ASIC1a; ASIC1b; B530003N02Rik; BNaC2
UniProt ID: (Human) P78348, (Rat) P55926, (Mouse) Q6NXK8
Entrez Gene ID: (Human) 41, (Rat) 79123, (Mouse) 11419
behavioral fear response
response to amphetamine
cation transport
sodium ion transport
memory
associative learning
response to acidic pH
ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
negative regulation of neurotransmitter secretion
sensory perception of sour taste
transmembrane transport
calcium ion transmembrane transport
cellular response to pH
regulation of postsynapse assembly
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support