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Invitrogen
ASPH Polyclonal Antibody
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FIGURE: 1 / 4
ASPH Antibody (PA5-97476) in ICC/IF
Immunofluorescent analysis of ASPH in Hela cells using a ASPH polyclonal antibody (Product # PA5-97476) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-97476
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Aspartyl/asparaginyl beta-hydroxylase protein (75-270AA)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins. This gene is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A beta H-J-J; ASP beta-hydroxylase; Aspartate beta-hydroxylase; aspartyl beta-hydroxylase; Aspartyl/asparaginyl beta-hydroxylase; aspartyl/asparaginyl-beta-hydroxylase; calsequestrin-binding protein; cardiac junctin; humbug; jumbug; junctate; junctin; Peptide-aspartate beta-dioxygenase
Gene Aliases: 2310005F16Rik; 3110001L23Rik; AAH; AI848629; ASPH; AW261690; AW561901; BAH; C79816; CASQ2BP1; cI-37; FDLAB; HAAH; JCTN; junctin
UniProt ID: (Human) Q12797, (Mouse) Q8BSY0
Entrez Gene ID: (Human) 444, (Mouse) 65973
muscle contraction
pattern specification process
negative regulation of cell proliferation
regulation of protein stability
ion transmembrane transport
limb morphogenesis
peptidyl-aspartic acid hydroxylation
positive regulation of proteolysis
oxidation-reduction process
palate development
face morphogenesis
activation of cysteine-type endopeptidase activity
regulation of protein depolymerization
regulation of cardiac conduction
positive regulation of calcium ion transport into cytosol
peptidyl-amino acid modification
regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
activation of store-operated calcium channel activity
response to ATP
positive regulation of transcription, DNA-templated
regulation of cytosolic calcium ion concentration
calcium ion transmembrane transport
cellular response to calcium ion
positive regulation of intracellular protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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