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ASS1 Monoclonal Antibody (1D1A11)

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Antibody Testing Data (13)

ASS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

ASS1 Antibody (66036-1-IG) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-IG at 1:50 dilution and Rhodamine-labeled goat anti-mouse IGG (red).

ASS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66036-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

Product Specifications

Species Reactivity

Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D1A11

Immunogen

ASS1 Fusion Protein Ag9314 (149-412 aa encoded by BC009243)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EFYNRFKGR NDLMEYAKQH GIPIPVTPKN PWSMDENLMH ISYEAGILEN PKNQAPPGLY TKTQDPAKAP NTPDILEIEF KKGVPVKVTN VKDGTTHQTS LELFMYLNEV AGKHGVGRID IVENRFIGMK SRGIYETPAG TILYHAHLDI EAFTMDREVR KIKQGLGLKF AELVYTGFWH SPECEFVRHC IAKSQERVEG KVQVSVLKGQ VYILGRESPL SLYNEELVSM NVQGDYEPTD ATGFININSL RLKEYHRLQS KVTAK (149-412 aa encoded by BC009243)

Target Information

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis.

Molecular pharmacology

Tu MJ,Duan Z,Liu Z,Zhang C,Bold RJ,Gonzalez FJ,Kim EJ,Yu AM

Tue Dec 01 00:00:00 EST 2020

Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.

BMC cancer

Feng Q,Bian X,Liu X,Wang Y,Zhou H,Ma X,Quan C,Yao Y,Zheng Z

Thu Jul 16 00:00:00 EDT 2020

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tsai CY,Chi HC,Chi LM,Yang HY,Tsai MM,Lee KF,Huang HW,Chou LF,Cheng AJ,Yang CW,Wang CS,Lin KH

Tue May 01 00:00:00 EDT 2018

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tsai CY,Chi HC,Chi LM,Yang HY,Tsai MM,Lee KF,Huang HW,Chou LF,Cheng AJ,Yang CW,Wang CS,Lin KH

Tue May 01 00:00:00 EDT 2018

Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-κB signaling.

BMC cancer

Liu J,Ma J,Wu Z,Li W,Zhang D,Han L,Wang F,Reindl KM,Wu E,Ma Q

Sat Sep 20 00:00:00 EDT 2014

Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-κB signaling.

BMC cancer

Liu J,Ma J,Wu Z,Li W,Zhang D,Han L,Wang F,Reindl KM,Wu E,Ma Q

Sat Sep 20 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Argininosuccinate synthase; argininosuccinate synthetase 1; Ass1; Citrulline--aspartate ligase; citrulline-aspartate ligase; RP11-618A20.2

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Gene Aliases: ASS; ASS1; CTLN1

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UniProt ID: (Human) P00966

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Entrez Gene ID: (Pig) 414411, (Human) 445

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Function(s)

argininosuccinate synthase activity protein binding ATP binding toxic substance binding amino acid binding identical protein binding poly(A) RNA binding ligase

Process(es)

Performance Guarantee

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ASS1 Monoclonal Antibody (1D1A11)

ASS1 Antibody (66036-1-IG) in ICC/IF

Product Details

66036-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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