|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide near the carboxy terminus of human ATG9B.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is Hela cell lysate.
PA5-20998 can be used with blocking peptide PEP-1112.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it and quote;s highly expressed in placenta and pituitary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors.
PA5-20998 was used in western blot to decipher activation of TFEB and Nrf2 transcription factors and stimulation by Fisetin for autophagic degradation of phosphorylated tau
|Kim S,Choi KJ,Cho SJ,Yun SM,Jeon JP,Koh YH,Song J,Johnson GV,Jo C||Scientific reports (6:null)||2016|