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Invitrogen
ATGL Polyclonal Antibody
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FIGURE: 1 / 6
ATGL Antibody (PA5-18672) in ICC/IF
Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATGL Polyclonal Antibody (Product # PA5-18672) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alex... View More
Product Details
PA5-18672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (NIIEVSKEARKR) corresponding to the internal amino acids of PNPLA2 (aa 70-80).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adipose triglyceride lipase; ATGL DESNUTRIN; calcium-independent phospholipase A2; Calcium-independent phospholipase A2-zeta; Desnutrin; DKFZp667M109; IPLA2 zeta; IPLA2-zeta; mutant patatin-like phospholipase domain containing 2; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain containing protein 2; Patatin-like phospholipase domain-containing protein 2; PEDF R; PEDF-R; pigment epithelium-derived factor; Pigment epithelium-derived factor receptor; plpl; plpl2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS 2.2; TTS2; TTS2.2
Gene Aliases: 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; PNPLA2; TTS-2.2; TTS2
UniProt ID: (Human) Q96AD5
Entrez Gene ID: (Human) 57104
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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