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Invitrogen
ATOH8 Polyclonal Antibody
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FIGURE: 1 / 2
ATOH8 Antibody (PA5-99779) in ICC/IF
Immunocytochemistry analysis of ATOH8 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATOH8 polyclonal antibody (Product # PA5-99779) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99779
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATOH8(Accession Q96SQ7), corresponding to amino acid residues Q264-G314.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ATOH8.
Target Information
Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: atonal homolog 6; atonal homolog 8; atonal homolog bHLH transcription factor 8; basic helix loop helix transcription factor 6; bHLHa21; Class A basic helix-loop-helix protein 21; Hath6; Helix-loop-helix protein hATH-6; Protein atonal homolog 8; Transcription factor ATOH8; unnamed protein product
Gene Aliases: ATH6; ATOH8; BHLHA21; HATH6
UniProt ID: (Human) Q96SQ7
Entrez Gene ID: (Human) 84913
formation of primary germ layer
negative regulation of endothelial cell proliferation
nervous system development
anatomical structure morphogenesis
positive regulation of endothelial cell migration
negative regulation of gene expression
cell differentiation
tube formation
positive regulation of endothelial cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
anatomical structure formation involved in morphogenesis
myoblast proliferation
positive regulation of SMAD protein import into nucleus
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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