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ATP citrate lyase Polyclonal Antibody

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ATP citrate lyase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ATP citrate lyase Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ATP citrate lyase Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug ATP citrate lyase antibody (15421-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

ATP citrate lyase Antibody in Immunoprecipitation (IP) — IP result of anti-ATP citrate lyase (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:1000) with K-562 cells lysate 3200ug.

Product Details

15421-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 13 publications 13 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP citrate lyase Fusion Protein Ag7709 (751-1101 aa encoded by BC006195) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MFSSEVQFG HAGACANQAS ETAVAKNQAL KEAGVFVPRS FDELGEIIQS VYEDLVANGV IVPAQEVPPP TVPMDYSWAR ELGLIRKPAS FMTSICDERG QELIYAGMPI TEVFKEEMGI GGVLGLLWFQ KRLPKYSCQF IEMCLMVTAD HGPAVSGAHN TIICARAGKD LVSSLTSGLL TIGDRFGGAL DAAAKMFSKA FDSGIIPMEF VNKMKKEGKL IMGIGHRVKS INNPDMRVQI LKDYVRQHFP ATPLLDYALE VEKITTSKKP NLILNVDGLI GVAFVDMLRN CGSFTREEAD EYIDIGALNG IFVLGRSMGF IGHYLDQKRL KQGLYRHPWD DISYVLPEHM SM (751-1101 aa encoded by BC006195)

Target Information

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.

Frontiers in immunology

Namgaladze D,Zukunft S,Schnütgen F,Kurrle N,Fleming I,Fuhrmann D,Brüne B

Fri Oct 25 00:00:00 EDT 2019

DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.

International journal of biological sciences

Xu M,Wu H,Li M,Wen Y,Yu C,Xia L,Xia Q,Kong X

Mon Sep 09 00:00:00 EDT 2019

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

Cell

Liu X,Cooper DE,Cluntun AA,Warmoes MO,Zhao S,Reid MA,Liu J,Lund PJ,Lopes M,Garcia BA,Wellen KE,Kirsch DG,Locasale JW

Thu Oct 04 00:00:00 EDT 2018

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

Cell

Liu X,Cooper DE,Cluntun AA,Warmoes MO,Zhao S,Reid MA,Liu J,Lund PJ,Lopes M,Garcia BA,Wellen KE,Kirsch DG,Locasale JW

Thu Oct 04 00:00:00 EDT 2018

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 EST 2017

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.

Molecular cell

Sivanand S,Rhoades S,Jiang Q,Lee JV,Benci J,Zhang J,Yuan S,Viney I,Zhao S,Carrer A,Bennett MJ,Minn AJ,Weljie AM,Greenberg RA,Wellen KE

Thu Jul 20 00:00:00 EDT 2017

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Nature

Mews P,Donahue G,Drake AM,Luczak V,Abel T,Berger SL

Thu Jun 15 00:00:00 EDT 2017

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Nature

Mews P,Donahue G,Drake AM,Luczak V,Abel T,Berger SL

Thu Jun 15 00:00:00 EDT 2017

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The EMBO journal

Wang L,Zhang T,Wang L,Cai Y,Zhong X,He X,Hu L,Tian S,Wu M,Hui L,Zhang H,Gao P

Mon May 15 00:00:00 EDT 2017

ACSS2-mediated acetyl-CoA synthesis from acetate is necessary for human cytomegalovirus infection.

Proceedings of the National Academy of Sciences of the United States of America

Vysochan A,Sengupta A,Weljie AM,Alwine JC,Yu Y

Tue Feb 21 00:00:00 EST 2017

The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.

Oncogene

Li G,Li M,Hu J,Lei R,Xiong H,Ji H,Yin H,Wei Q,Hu G

Thu Feb 16 00:00:00 EST 2017

Acetyl-coenzyme A carboxylase alpha promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Hepatology (Baltimore, Md.)

Wang MD,Wu H,Fu GB,Zhang HL,Zhou X,Tang L,Dong LW,Qin CJ,Huang S,Zhao LH,Zeng M,Wu MC,Yan HX,Wang HY

Fri Apr 01 00:00:00 EDT 2016

Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma.

International journal of molecular sciences

Sun H,Zhu A,Zhang L,Zhang J,Zhong Z,Wang F

Thu Oct 15 00:00:00 EDT 2015

Mutations in CIC and IDH1 cooperatively regulate 2-hydroxyglutarate levels and cell clonogenicity.

Oncotarget

Chittaranjan S,Chan S,Yang C,Yang KC,Chen V,Moradian A,Firme M,Song J,Go NE,Blough MD,Chan JA,Cairncross JG,Gorski SM,Morin GB,Yip S,Marra MA

Mon Sep 15 00:00:00 EDT 2014

Mutations in CIC and IDH1 cooperatively regulate 2-hydroxyglutarate levels and cell clonogenicity.

Oncotarget

Chittaranjan S,Chan S,Yang C,Yang KC,Chen V,Moradian A,Firme M,Song J,Go NE,Blough MD,Chan JA,Cairncross JG,Gorski SM,Morin GB,Yip S,Marra MA

Mon Sep 15 00:00:00 EDT 2014

Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1.

Journal of virology

Yu Y,Maguire TG,Alwine JC

Thu Mar 01 00:00:00 EST 2012

Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1.

Journal of virology

Yu Y,Maguire TG,Alwine JC

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme

Gene Aliases: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP

UniProt ID: (Human) P53396, (Mouse) Q91V92, (Rat) P16638

Entrez Gene ID: (Human) 47, (Mouse) 104112, (Rat) 24159

Molecular Function: transferase

Function(s)

acetyl-CoA biosynthetic process citrate metabolic process oxaloacetate metabolic process fatty acid biosynthetic process cholesterol biosynthetic process lipid biosynthetic process positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process acetyl-CoA metabolic process lipid metabolic process metabolic process cellular carbohydrate metabolic process

Process(es)

ATP citrate synthase activity protein binding ATP binding metal ion binding cofactor binding nucleotide binding catalytic activity succinate-CoA ligase (ADP-forming) activity transferase activity transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer lyase activity

ATP citrate lyase Polyclonal Antibody

Product Details

15421-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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