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ATP1A1 Polyclonal Antibody

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ATP1A1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (Product # 14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14418-1-AP ( ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

ATP1A1 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ATP1A1 Antibody in Immunoprecipitation (IP) — IP result of anti-ATP1A1 (IP:14418-1-AP, 4 ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200 ug.

Product Details

14418-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 21 publications 21 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP1A1 Fusion Protein Ag5763 (347-680 aa encoded by BC050359) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TAKRMARKN CLVKNLEAVE TLGSTSTICS DKTGTLTQNR MTVAHMWFDN QIHEADTTEN QSGVSFDKTS ATWLALSRIA GLCNRAVFQA NQENLPILKR AVAGDASESA LLKCIELCCG SVKEMRERYA KIVEIPFNST NKYQLSIHKN PNTSEPQHLL VMKGAPERIL DRCSSILLHG KEQPLDEELK DAFQNAYLEL GGLGERVLGF CHLFLPDEQF PEGFQFDTDD VNFPIDNLCF VGLISMIDPP RAAVPDAVGK CRSAGIKVIM VTGDHPITAK AIAKGVGIIS EGNETVEDIA ARLNIPVSQV NPRDAKACVV HGSDLKDMTS EQLDD (347-680 aa encoded by BC050359)

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium-transporting ATPase subunit alpha-1

Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b

UniProt ID: (Human) P05023, (Rat) P06685, (Mouse) Q8VDN2

Entrez Gene ID: (Human) 476, (Rat) 24211, (Mouse) 11928

Molecular Function: primary active transporter