Invitrogen

ATP6V0D1 Polyclonal Antibody

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ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ATP6V0D1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ATP6V0D1 polyclonal antibody (Product # PA5-103179) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ATP6V0D1 Antibody in Western Blot (WB) — Western blot analysis of ATP6V0D1 in HeLa cell lysate. Samples were incubated with ATP6V0D1 polyclonal antibody (Product # PA5-103179).

Product Details

PA5-103179

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunocytochemistry (ICC/IF)	1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ATP6V0D1(Accession P61421), corresponding to amino acid residues Y271-V299. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2852548

Target Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 32 kDa accessory protein; ATPase, H+ transporting, lysosomal (vacuolar proton pump), 42 kDa; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1; ATPase, H+ transporting, V0 subunit D; FLJ43534; H(+)-transporting two-sector ATPase, subunit D; lysosomal 38kDa; P39; Physophilin; V-ATPase 40 KDa accessory protein; V-ATPase AC39 subunit; V-ATPase subunit d 1; V-ATPase, subunit D; V-type proton ATPa; V-type proton ATPase subunit d 1; Vacuolar proton pump subunit d 1

Gene Aliases: Ac39; AI267038; ATP6D; ATP6DV; ATP6V0D1; P39; VATX; VMA6; VPATPD

UniProt ID: (Human) P61421, (Mouse) P51863

Entrez Gene ID: (Human) 9114, (Rat) 291969, (Mouse) 11972

Molecular Function: ATP synthase