Invitrogen

ATP6V0D1 Polyclonal Antibody

View all (6) ATP6V0D1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

ATP6V0D1 Antibody (PA5-103179) in ICC/IF

Immunofluorescent analysis of ATP6V0D1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ATP6V0D1 polyclonal antibody (Product # PA5-103179) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Product Image Gallery

ATP6V0D1 Antibody in Western Blot (WB) — Western blot analysis of ATP6V0D1 in HeLa cell lysate. Samples were incubated with ATP6V0D1 polyclonal antibody (Product # PA5-103179).

Product Details

PA5-103179

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ATP6V0D1(Accession P61421), corresponding to amino acid residues Y271-V299.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2852548

Target Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 32 kDa accessory protein; ATPase, H+ transporting, lysosomal (vacuolar proton pump), 42 kDa; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1; ATPase, H+ transporting, V0 subunit D; FLJ43534; H(+)-transporting two-sector ATPase, subunit D; lysosomal 38kDa; P39; Physophilin; V-ATPase 40 KDa accessory protein; V-ATPase AC39 subunit; V-ATPase subunit d 1; V-ATPase, subunit D; V-type proton ATPa; V-type proton ATPase subunit d 1; Vacuolar proton pump subunit d 1

Gene Aliases: Ac39; AI267038; ATP6D; ATP6DV; ATP6V0D1; P39; VATX; VMA6; VPATPD

UniProt ID: (Human) P61421, (Mouse) P51863

Entrez Gene ID: (Human) 9114, (Rat) 291969, (Mouse) 11972

Molecular Function: ATP synthase