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ATP6V1B1 Antibody (PA5-35052) in ICC/IF
Immunocytochemistry analysis of ATP6V1B1 in WiDr cells. Samples were incubated in ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Details
PA5-35052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 284-310 amino acids from the central region of human ATP6V1B1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552362
Product Specific Information
This antibody is predicted to react with bovine, chicken, Drosophila, C. elegans, mouse and rat based on sequence homology.
Target Information
Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; Endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; V-ATPase B1; V-ATPase B1 subunit; V-ATPase subunit B 1; V-type proton ATPase subunit B, kidney isoform; vacuolar proton pump 3; Vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3
Gene Aliases: ATP6B1; ATP6V1B1; RTA1B; VATB; VMA2; VPP3
UniProt ID: (Human) P15313
Entrez Gene ID: (Human) 525
ossification
regulation of pH
excretion
sensory perception of sound
insulin receptor signaling pathway
ATP hydrolysis coupled proton transport
proton transport
regulation of macroautophagy
transferrin transport
ion transmembrane transport
inner ear morphogenesis
pH reduction
ATP metabolic process
calcium ion homeostasis
phagosome acidification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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