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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 68-339 of human AUH (NP_001689.1). |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
3.16 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2854845 |
Sequence of this protein is as follows: SSEMKTEDEL RVRHLEEENR GIVVLGINRA YGKNSLSKNL IKMLSKAVDA LKSDKKVRTI IIRSEVPGIF CAGADLKERA KMSSSEVGPF VSKIRAVIND IANLPVPTIA AIDGLALGGG LELALACDIR VAASSAKMGL VETKLAIIPG GGGTQRLPRA IGMSLAKELI FSARVLDGKE AKAVGLISHV LEQNQEGDAA YRKALDLARE FLPQGPVAMR VAKLAINQGM EVDLVTGLAI EEACYAQTIP TKDRLEGLLA FKEKRPPRYK GE
The methylglutaconyl-CoA hydratase, mitochondrial protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3-methylglutaconyl-CoA hydratase; AU RNA binding protein/enoyl-coenzyme A hydratase; AU RNA-binding protein/enoyl-Coenzyme A hydratase; AU-binding enoyl-CoA hydratase; AU-binding protein/Enoyl-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; Itaconyl-CoA hydratase; Methylglutaconyl-CoA hydratase, mitochondrial; muAUH
Gene Aliases: AUH; C77140; W91705
UniProt ID: (Human) Q13825, (Rat) F1LU71, (Mouse) Q9JLZ3
Entrez Gene ID: (Human) 549, (Rat) 361215, (Mouse) 11992
Molecular Function:
hydratase
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