Invitrogen

AXIN1 Polyclonal Antibody

Name: AXIN1 Polyclonal Antibody
Brand: Invitrogen
SKU: 34-5900

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4 Published Figures

13 References

View all (28) AXIN1 antibodies

Datasheet

Certificates

Protocols

Datasheet

Certificates

Protocols

Datasheet

Certificates

Protocols

Additional Information:

FIGURE: 1 / 6

AXIN1 Antibody (34-5900) in WB

Western blot analysis of Jurkat cell lysates using Axin Polyclonal Antibody, Rabbit (Product # 34-5900).

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AXIN1 Antibody — Figure 4 Forskolin reduced the expression of beta-catenin through upregulation of the expression of Axin. Notes: ( A, B ) The expressions of Axin, Frz, APC, GSK-3beta, and CK1 in forskolin (40 muM)-treated or control Toledo and NK-92 cells were examined by Western blotting analysis (n=3, * P <0.05, forskolin group vs control group). ( C, D ) RT-PCR and Western blotting were carried out to detect the mRNA and protein levels of Axin after Toledo or NK-92 cells were transfected with si-1/2/3 targeting Axin gene for 24 or 48 hours, respectively (n=3, * P <0.05, *** P <0.001, si-1/2/3 group vs si-NC group). ( E ) Western blotting analysis of the expression of beta-catenin in different groups of Toledo and NK-92 cells: si-NC, si-NC + forskolin, si-Axin, si-Axin + forskolin (n=3, *** P <0.001, si-NC + forskolin group or si-Axin group vs control group). ( F, G ) The protein expression of cytoplasmic and nuclear beta-catenin in Toledo and NK-92 cells with different treatments: si-NC, si-NC + forskolin, si-Axin, si-Axin + forskolin (n=3, * P <0.05, si-NC + forskolin group or si-Axin group vs control group). Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GSK-3beta, glycogen synthase kinase 3beta. {TM}

Product Details

34-5900

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Fruit fly, Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2533178

Target Information

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a disheveled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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AXIN1 Polyclonal Antibody

AXIN1 Antibody (34-5900) in WB

Product Details

34-5900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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