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Alomone Labs, LTD
AXL (extracellular) Polyclonal Antibody, FITC
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FIGURE: 1 / 3
AXL (extracellular) Antibody (ATR-032-F-50UL) in Flow
Cell surface detection of AXL by direct flow cytometry in live intact human THP-1 monocytic leukemia cells. Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-AXL (extracellular)-FITC Antibody (#ATR-032-F) (5 µg).
Product Details
ATR-032-F-50UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide GNDDLYAHHSTAR, corresponding to amino acid residues 18-31 of human GPR183. Extracellular, N-terminus.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunogen sequence: GNDDLYAHHSTAR
Reconstitution: 25 uL, 50 uL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion-related kinase; AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO; ufo oncogene homolog
Gene Aliases: AI323647; ARK; AXL; JTK11; Tyro7; UFO
UniProt ID: (Human) P30530, (Mouse) Q00993
Entrez Gene ID: (Human) 558, (Mouse) 26362, (Rat) 308444
virus receptor activity
phosphatidylserine binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
ATP binding
protein heterodimerization activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
myosin heavy chain binding
phosphatidylinositol 3-kinase binding
growth factor binding
transmembrane signal receptor
neuron migration
natural killer cell differentiation
positive regulation of cytokine-mediated signaling pathway
blood vessel remodeling
phagocytosis
inflammatory response
signal transduction
spermatogenesis
peptidyl-tyrosine phosphorylation
forebrain cell migration
platelet activation
cellular response to extracellular stimulus
negative regulation of interferon-gamma production
negative regulation of tumor necrosis factor production
positive regulation of natural killer cell differentiation
secretion by cell
erythrocyte homeostasis
substrate adhesion-dependent cell spreading
cellular response to interferon-alpha
ovulation cycle
apoptotic cell clearance
protein kinase B signaling
negative regulation of neuron apoptotic process
innate immune response
viral entry into host cell
vascular endothelial growth factor receptor signaling pathway
cell maturation
positive regulation of pinocytosis
negative regulation of lymphocyte activation
positive regulation of protein kinase B signaling
vagina development
cellular response to lipopolysaccharide
dendritic cell differentiation
negative regulation of dendritic cell apoptotic process
immune system process
protein phosphorylation
enzyme linked receptor protein signaling pathway
phosphorylation
cell differentiation
negative regulation of apoptotic process
cellular response to hydrogen peroxide
cell proliferation
cell migration
organ regeneration
tissue regeneration
tissue remodeling
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