This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
701269 was successfully used to detect H3K9ac in WB, IF, IHC, ChIP and flow applications.
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Histone H3 is one of the four core proteins of the nucleosome, and it is involved in transcription regulation, DNA repair, DNA replication and chromosomal stability. The N-terminal tail of Histone H3 undergoes many post-translational modifications, including phosphorylation, acetylation, multiple methylation, ubiquitination, and ADP-ribosylation to achieve its diverse functions. HistoneH3 is acetylated and deacetylated on N-terminal lysine residues. Acetylation removes the positive charge on the histone, decreasing the interaction with the negatively charged phosphate groups of DNA, and resulting in a more relaxed structure associated with greater levels of gene transcription. Acetylation of histone H3 at lysine 9 (H3K9Ac) is one of the most well-known epigenetic markers enriched in the promoter region of activated genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H3 histone family, member A; H3a; H3K9ac; HIST1H3A; Histone 1; histone 1, H3a; histone cluster 1, H3a; histone cluster 3, H3; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Gene Aliases: H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J