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Western blot analysis of Aconitase 1 using A) 30 µg 293T whole cell lysate (B) 30 µg HeLa whole cell lysate and C) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Aconitase 1 polyclonal antibody (Product # PA5-27824) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 83 and 461 of Human Aconitase 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27824 targets Aconitase 1 in WB applications and shows reactivity with Human samples.
The PA5-27824 immunogen is recombinant fragment corresponding to a region within amino acids 83 and 461 of Human Aconitase 1.
Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
aconitase 1, soluble; aconitate hydratase; aconitate hydratase, cytoplasmic; ACONS; citrate hydro-lyase; cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; ferritin repressor protein; IRE-BP 1; IREB1; IREBP; IREBP1; iron regulatory protein 1; iron-responsive element binding protein 1; iron-responsive element-binding protein 1; IRP1; soluble aconitase
ACO1; ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1