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Aconitase 1 Antibody (PA5-77845) in IHC (P)
Aconitase 1 Polyclonal Antibody detects Aconitase 1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Aconitase 1 Polyclonal Antibody (Product # PA5-77845) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-77845
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Aconitase 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735425
Product Specific Information
Positive Control: A431, HeLa, HepG2
Predicted Reactivity: Mouse (87%), Rat (86%), Rabbit (90%), Rhesus Monkey (99%), Bovine (89%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Aconitase 1 (ACO1) also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aconitase; aconitase 1, soluble; aconitate hydratase, cytoplasmic; Citrate hydro-lyase; Cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; Ferritin repressor protein; IRE-BP 1; Iron regulatory protein 1; iron-responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP1; OTTHUMP00000021176; OTTHUMP00000021177; OTTHUMP00000045233; soluble aconitase
Gene Aliases: ACO1; ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
UniProt ID: (Human) P21399
Entrez Gene ID: (Human) 48
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