Invitrogen

Adenosine Kinase Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

4 References

View all (18) ADK antibodies

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Datasheet

Protocols

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Adenosine Kinase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Adenosine Kinase was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Adenosine Kinase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Adenosine Kinase Polyclonal Antibody detects Adenosine kinase protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Adenosine Kinase Antibody in Western Blot (WB) — Knockdown of Adenosine Kinase was achieved by transfecting A-431 with Adenosine Kinase specific siRNAs (Silencer® select Product # s1081, s1080). Western blot analysis (Fig. a) was performed using whole cell extracts from the Adenosine Kinase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Adenosine Kinase Polyclonal Antibody (Product # PA5-27399, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Adenosine Kinase.

Adenosine Kinase Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A431 cells were transfected with Adenosine Kinase siRNA and reduction of signal was observed in Western Blot using Adenosine Kinase Polyclonal Antibody (Product # PA5-27399). {KD}

Product Details

PA5-27399

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 146 and 362 of Human Adenosine kinase View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.28 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544875

Product Specific Information

Recommended positive controls: A431, HepG2, HeLa, mouse liver, rat liver.

Predicted reactivity: Mouse (89%), Rat (87%), Chicken (83%), Rhesus Monkey (99%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of Adenosine Kinase in Sphingosine-1-Phosphate Receptor 1-Induced Mechano-Hypersensitivities.

Cellular and molecular neurobiology

Lauro F,Giancotti LA,Kolar G,Harada CM,Harmon TA,Garrett TJ,Salvemini D

PA5-27399 was used in Western Blot to show that SEW287-induced behavioral hypersensitivity is associated with increased expression of ADK in astrocytes of the dorsal horn of the spinal cord.

Tue Nov 01 00:00:00 EDT 2022

Adenosine Kinase Isoforms in the Developing Rat Hippocampus after LiCl/Pilocarpine Status Epilepticus.

International journal of molecular sciences

Fábera P,Uttl L,Kubová H,Tsenov G,Mareš P

Published figure using Adenosine Kinase polyclonal antibody (Product # PA5-27399) in Western Blot

Thu Feb 24 00:00:00 EST 2022

Chronic Morphine-Induced Changes in Signaling at the A₃ Adenosine Receptor Contribute to Morphine-Induced Hyperalgesia, Tolerance, and Withdrawal.

The Journal of pharmacology and experimental therapeutics

Doyle TM,Largent-Milnes TM,Chen Z,Staikopoulos V,Esposito E,Dalgarno R,Fan C,Tosh DK,Cuzzocrea S,Jacobson KA,Trang T,Hutchinson MR,Bennett GJ,Vanderah TW,Salvemini D

PA5-27399 was used in Western Blotting to demonstrate that opioid-induced hyperalgesia and antinociceptive tolerance in rats is associated with a twofold increase in adenosine kinase expression in the dorsal horn of the spinal cord.

Sat Aug 01 00:00:00 EDT 2020

Chemotherapy-induced pain is promoted by enhanced spinal adenosine kinase levels through astrocyte-dependent mechanisms.

Pain

Wahlman C,Doyle TM,Little JW,Luongo L,Janes K,Chen Z,Esposito E,Tosh DK,Cuzzocrea S,Jacobson KA,Salvemini D

PA5-27399 was used in Western Blotting to strengthen the pharmacological rationale for clinical evaluation of A3AR agonists already in advanced clinical trials as anticancer agents as an adjunct to chemotherapy.

Fri Jun 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Adenosine 5'-phosphotransferase; Adenosine kinase; AK; testicular tissue protein Li 14

Gene Aliases: 2310026J05Rik; 5033405D03Rik; ADK; AI255373; AI987814; AK

UniProt ID: (Human) P55263, (Mouse) P55264, (Rat) Q64640

Entrez Gene ID: (Human) 132, (Mouse) 11534, (Rat) 25368

Function(s)

purine ribonucleoside salvage AMP biosynthetic process dATP biosynthetic process positive regulation of cardiac muscle hypertrophy phosphorylation positive regulation of T cell proliferation type B pancreatic cell proliferation adenosine metabolic process circadian regulation of gene expression AMP salvage ribonucleoside monophosphate biosynthetic process purine-containing compound salvage

Process(es)

adenosine kinase activity ATP binding poly(A) RNA binding metal ion binding nucleotide binding kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor

Adenosine Kinase Polyclonal Antibody

Product Details

PA5-27399

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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