Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Adiponectin in whole cell extracts from serum-starved 3T3 L1 cells treated with Insulin (100ng/ml, 15 min) using an Adiponectin recombinant rabbit oligoclonal antibody (Product # 710179) at a dilution of 1ug/ml. Samples were detected using chemiluminescence (ECL). Results show a band at ~28kDa.
|Tested species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 57–244 of human adiponectin|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Adiponectin is a hormone exclusively produced in adipose tissue and secreted into plasma. It is involved in a number of metabolic processes, such as glucose regulation, and the metabolism of fat for energy production. Adiponectin is involved in the control of fat metabolism and insulin sensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
30kDa, ADIPQTL1, ADPN, adipo, ACRP30, Acdc, apM1, GBP28, APN, ACDC, Ad, APM-1, Acrp30, APM1
30 kDa adipocyte complement-related protein, adipocyte complement-related 30 kDa protein, adiponectin, adipose most abundant gene transcript 1 protein, adipose specific collagen-like factor, gelatin-binding protein 28, adipocyte complement related protein, adipocyte, C1Q and collagen domain containing, adipocyte, C1q and collagen domain-containing protein, adipocyte-specific protein AdipoQ, Adipocyte, C1q and collagen domain-containing protein, ACRP30, apM-1, GBP28, APM1, ACDC, ADIPOQ