Proteintech

Alpha-1-Antitrypsin Polyclonal Antibody

Alpha-1-Antitrypsin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Alpha-1-Antitrypsin Antibody (16382-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 16382-1-AP) Alpha-1-Antitrypsin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha-1-Antitrypsin Antibody in Western Blot (WB)

Alpha-1-Antitrypsin Antibody in Flow Cytometry (Flow)

Alpha-1-Antitrypsin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16382-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alpha-1-Antitrypsin Fusion Protein Ag9369 (28-418 aa encoded by BC015642)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.48 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QGDAAQKTD TSHHDQDHPT FNKITPNLAE FAFSLYRQLA HQSNSTNIFF SPVSIATAFA MLSLGTKADT HDEILEGLNF NLTEIPEAQI HEGFQELLRT LNQPDSQLQL TTGNGLFLSE GLKLVDKFLE DVKKLYHSEA FTVNFGDTEE AKKQINDYVE KGTQGKIVDL VKELDRDTVF ALVNYIFFKG KWERPFEVKD TEEEDFHVDQ ATTVKVPMMK RLGMFNIQHC KKLSSWVLLM KYLGNATAIF FLPDEGKLQH LENELTHDII TKFLENEDRR SASLHLPKLS ITGTYDLKSV LGQLGITKVF SNGADLSGVT EEAPLKLSKA VHKAVLTIDE KGTEAAGAMF LEAIPMSIPP EVKFNKPFVF LMIEQNTKSP LFMGKVVNPT QK (28-418 aa encoded by BC015642)

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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The Journal of nutritional biochemistry

Yang J,Zhi Y,Wen S,Pan X,Wang H,He X,Lu Y,Zhu Y,Chen Y,Shi G

Sun Jan 01 00:00:00 UTC 2023

Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells.

International journal of molecular sciences

Muranaka M,Takamatsu S,Ouchida T,Kanazawa Y,Kondo J,Nakagawa T,Egashira Y,Fukagawa K,Gu J,Okamoto T,Kamada Y,Miyoshi E

Tue Apr 11 00:00:00 UTC 2023

Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity.

Cell reports

Kaserman JE,Werder RB,Wang F,Matte T,Higgins MI,Dodge M,Lindstrom-Vautrin J,Bawa P,Hinds A,Bullitt E,Caballero IS,Shi X,Gerszten RE,Brunetti-Pierri N,Liesa M,Villacorta-Martin C,Hollenberg AN,Kotton DN,Wilson AA

Tue Dec 06 00:00:00 UTC 2022

Matrix metalloproteinase-13 is fully activated by neutrophil elastase and inactivates its serpin inhibitor, alpha-1 antitrypsin: Implications for osteoarthritis.

The FEBS journal

Wilkinson DJ,Falconer AMD,Wright HL,Lin H,Yamamoto K,Cheung K,Charlton SH,Arques MDC,Janciauskiene S,Refaie R,Rankin KS,Young DA,Rowan AD

Sat Jan 01 00:00:00 UTC 2022

New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation.

Nutrients

Hu PA,Wang SH,Chen CH,Guo BC,Huang JW,Lee TS

Wed Jun 01 00:00:00 UTC 2022

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Matrix biology : journal of the International Society for Matrix Biology

Ouni E,Nedbal V,Da Pian M,Cao H,Haas KT,Peaucelle A,Van Kerk O,Herinckx G,Marbaix E,Dolmans MM,Tuuri T,Otala M,Amorim CA,Vertommen D

Sun May 01 00:00:00 UTC 2022

Targeting the Apoa1 locus for liver-directed gene therapy.

Molecular therapy. Methods & clinical development

De Giorgi M,Li A,Hurley A,Barzi M,Doerfler AM,Cherayil NA,Smith HE,Brown JD,Lin CY,Bissig KD,Bao G,Lagor WR

Fri Jun 11 00:00:00 UTC 2021

Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury.

PloS one

Stearns K,Goldklang M,Xiao R,Zelonina T,Blomenkamp K,Teckman J,D'Armiento JM

Fri Jul 23 00:00:00 UTC 2021

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Translational andrology and urology

Li X,Huang C,Zhang X,Yang T,Zuo S,Fu C,Zhang Y,Yang C,Chen L

Sun Aug 01 00:00:00 UTC 2021

Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.

Nature communications

Wettstein L,Weil T,Conzelmann C,Müller JA,Groß R,Hirschenberger M,Seidel A,Klute S,Zech F,Prelli Bozzo C,Preising N,Fois G,Lochbaum R,Knaff PM,Mailänder V,Ständker L,Thal DR,Schumann C,Stenger S,Kleger A,Lochnit G,Mayer B,Ruiz-Blanco YB,Hoffmann M,Sparrer KMJ,Pöhlmann S,Sanchez-Garcia E,Kirchhoff F,Frick M,Münch J

Fri Mar 19 00:00:00 UTC 2021

Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression.

Blood advances

Everett LA,Khoriaty RN,Zhang B,Ginsburg D

Tue Nov 24 00:00:00 UTC 2020

Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.

OncoTargets and therapy

Wu DM,Liu T,Deng SH,Han R,Zhang T,Li J,Xu Y

Thu Apr 14 00:00:00 UTC 2022

Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.

OncoTargets and therapy

Wu DM,Liu T,Deng SH,Han R,Zhang T,Li J,Xu Y

Thu Apr 14 00:00:00 UTC 2022

Proteomic analysis reveals the protective effects of emodin on severe acute pancreatitis induced lung injury by inhibiting neutrophil proteases activity.

Journal of proteomics

Xu C,Zhang J,Liu J,Li Z,Liu Z,Luo Y,Xu Q,Wang M,Zhang G,Wang F,Chen H

Sat May 30 00:00:00 UTC 2020

Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.

Molecular reproduction and development

An T,Wang YF,Liu JX,Pan YY,Liu YF,He ZC,Mo FF,Li J,Kang LH,Gu YJ,Lv BH,Gao SH,Jiang GJ

Mon Jan 01 00:00:00 UTC 2018

Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.

Molecular reproduction and development

An T,Wang YF,Liu JX,Pan YY,Liu YF,He ZC,Mo FF,Li J,Kang LH,Gu YJ,Lv BH,Gao SH,Jiang GJ

Mon Jan 01 00:00:00 UTC 2018

Free circulating active elastase contributes to chronic inflammation in patients on hemodialysis.

American journal of physiology. Renal physiology

Khatib-Massalha E,Michelis R,Trabelcy B,Gerchman Y,Kristal B,Ariel A,Sela S

Thu Feb 01 00:00:00 UTC 2018

Free circulating active elastase contributes to chronic inflammation in patients on hemodialysis.

American journal of physiology. Renal physiology

Khatib-Massalha E,Michelis R,Trabelcy B,Gerchman Y,Kristal B,Ariel A,Sela S

Thu Feb 01 00:00:00 UTC 2018

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Journal of proteomics

Jiao F,Zhang D,Jiang M,Mi J,Liu X,Zhang H,Hu Z,Xu X,Hu X

Fri Jan 06 00:00:00 UTC 2017

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Journal of proteomics

Jiao F,Zhang D,Jiang M,Mi J,Liu X,Zhang H,Hu Z,Xu X,Hu X

Fri Jan 06 00:00:00 UTC 2017

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Journal of proteomics

Jiao F,Zhang D,Jiang M,Mi J,Liu X,Zhang H,Hu Z,Xu X,Hu X

Fri Jan 06 00:00:00 UTC 2017

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Journal of proteomics

Jiao F,Zhang D,Jiang M,Mi J,Liu X,Zhang H,Hu Z,Xu X,Hu X

Fri Jan 06 00:00:00 UTC 2017

Surveying the serologic proteome in a tissue-specific kras(G12D) knockin mouse model of pancreatic cancer.

Proteomics

Ludwig MR,Kojima K,Bowersock GJ,Chen D,Jhala NC,Buchsbaum DJ,Grizzle WE,Klug CA,Mobley JA

Mon Feb 01 00:00:00 UTC 2016

Surveying the serologic proteome in a tissue-specific kras(G12D) knockin mouse model of pancreatic cancer.

Proteomics

Ludwig MR,Kojima K,Bowersock GJ,Chen D,Jhala NC,Buchsbaum DJ,Grizzle WE,Klug CA,Mobley JA

Mon Feb 01 00:00:00 UTC 2016

The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.

Nature communications

Mobin MB,Gerstberger S,Teupser D,Campana B,Charisse K,Heim MH,Manoharan M,Tuschl T,Stoffel M

Mon Sep 26 00:00:00 UTC 2016

The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.

Nature communications

Mobin MB,Gerstberger S,Teupser D,Campana B,Charisse K,Heim MH,Manoharan M,Tuschl T,Stoffel M

Mon Sep 26 00:00:00 UTC 2016

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Disease markers

He T,Hu JY,Han J,Zhang DX,Jiang XP,Chen B,Huang YS

Mon Jan 19 00:00:00 UTC 2015

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Disease markers

He T,Hu JY,Han J,Zhang DX,Jiang XP,Chen B,Huang YS

Mon Jan 19 00:00:00 UTC 2015

High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.

Medical oncology (Northwood, London, England)

Qin X,Chen Q,Sun C,Wang C,Peng Q,Xie L,Liu Y,Li S

Fri Mar 01 00:00:00 UTC 2013

High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.

Medical oncology (Northwood, London, England)

Qin X,Chen Q,Sun C,Wang C,Peng Q,Xie L,Liu Y,Li S

Fri Mar 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: a-1 AT; a1-antitrypsin; AAT; alpha 1 antitrypsin; alpha-1 antitrypsin; Alpha-1 protease inhibitor; Alpha-1 protease inhibitor 1; Alpha-1-antiproteinase; Alpha-1-antitrypsin; alpha-1-antitrypsin (protease inhibitor); Alpha-1-antitrypsin 1-1; alpha-1-antitrypsin 2; alpha-1-antitrypsin null; alpha-1-protease inhibitor; Alpha-1-proteinase inhibitor; Digestive Zymogen; MGC23330; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; Protease Serine 1; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1a; Serine Protease 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; serine protease inhibitor alpha 1; Serpin A1; Serpin A1a; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; TRP1; TRY1

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Gene Aliases: A1A; A1AT; AAT; Aat-2; Aat2; alpha1AT; Dom1; PI; PI1; PRO0684; PRO2209; PRO2275; SERPINA1; Serpina1a; Spi1; Spi1-1; Spi1-3

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UniProt ID: (Human) P01009, (Rat) P17475, (Mouse) P07758

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Entrez Gene ID: (Human) 5265, (Rat) 24648, (Mouse) 20700

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Function(s)

glycoprotein binding protease binding serine-type endopeptidase inhibitor activity protein binding identical protein binding endopeptidase inhibitor activity peptidase inhibitor activity protease inhibitor

Process(es)

platelet degranulation ER to Golgi vesicle-mediated transport acute-phase response blood coagulation negative regulation of endopeptidase activity COPII vesicle coating response to hypoxia inflammatory response response to inorganic substance response to lead ion response to organic cyclic compound response to estradiol response to lipopolysaccharide response to methanol response to triglyceride response to cytokine response to chromate negative regulation of serine-type endopeptidase activity protein N-linked glycosylation negative regulation of peptidase activity response to peptide hormone

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Alpha-1-Antitrypsin Polyclonal Antibody

Alpha-1-Antitrypsin Antibody (16382-1-AP) in IHC (P)

Product Details

16382-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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