This antibody is specific for the Annexin I protein and does not cross-react with other Annexins.Reactivity with Annexin I was confirmed by western blotting using total lysates derived from human fibroblasts. Positive control lysates used: human fibroblasts, mouse kidney, mouse lung, adult rat and mouse brain.
The Annexins are a structurally related family of calcium-dependent binding proteins that associate reversibly with membranes, and bind preferentially to the cytosolic face of phospholipid bilayers in the presence of micromolar-free calcium concentrations. This binding is specific for calcium and for acidic phospholipids. Each of the annexin proteins consists of an N-terminal domain of variable length followed by four or eight copies of a conserved segment of sixty-one residues. The repeat (sometimes known as an 'endonexin fold') consists of five alpha-helices that are wound into a right-handed superhelix. Annexins have a molecular weight of approximately 35,000 to 40,000; Annexin 1 has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins, and leukotrienes, annexin I may have potential anti-inflammatory activity. Annexins have also demonstrated to be involved in cytoskeletal interactions, intracellular signaling, anticoagulation, and membrane fusion. Although the annexins are primarily intracellular proteins and lack hydrophobic signal sequences, some family members (e.g., Annexin I and V) have been found to be secreted. Annexin I (also known as calpactin II, chromobindin 9, GIF, Lipocortin I, and p35) has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth-regulated tyrosine kinases, and may be involved in secretion. Although this protein has received considerable attention due to its potential involvement in a variety of pathways, research progress on the localization of Annexin I during development and in pathophysiological conditions has been hampered due to conflicting reports on the immunolocalization of the protein. These contradictory data are believed to result in large part from unusual properties of the protein and its apparent sensitivity to different fixation conditions. In addition to Annexin I, examples of other proteins known to belong to the annexin family include: .Annexin II (lipocortin 2, calpactin 1, protein I, p36, chromobindin 8), Annexin III (lipocortin 3, PAP-III), Annexin IV (lipocortin 4, endonexin I, protein II, chromobindin 4 ), Annexin V (Lipocortin 5, Endonexin 2, VAC-alpha, Anchorin CII, PAP-I), Annexin VI (Lipocortin 6, Protein III, Chromobindin 20, p68, p70), Annexin VII (Synexin), Annexin VIII (VAC-beta), Annexin XI (CAP-50), and Annexin XIII (ISA).
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Protein Aliases: Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; ANXA1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; p35; Phospholipase A2 inhibitory protein
Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35