Invitrogen

Apolipoprotein H Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (32) Apolipoprotein H antibodies

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Protocols

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Apolipoprotein H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Apolipoprotein H Polyclonal Antibody detects Apolipoprotein H protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Apolipoprotein H stained by Apolipoprotein H Polyclonal Antibody (Product # PA5-85389) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Apolipoprotein H Antibody in Western Blot (WB) — Western Blot was performed using Anti-Apolipoprotein H Polyclonal Antibody (Product # PA5-85389) and a 50 kDa band corresponding to Beta-2-glycoprotein 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 (Lane 2), PC-3 (Lane 3), PC-3 (Lane 4), BeWo (Lane 5), BeWo (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Apolipoprotein H Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Beta-2-glycoprotein 1 was observed in to be high in Hep G2 upon treatment with the protein secretory blocker in comparison to no expression in PC-3 and BeWo upon treatment with the protein secretory blocker using Anti-Apolipoprotein H Polyclonal Antibody (Product # PA5-85389) in Western Blot. {RE}

Product Details

PA5-85389

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human Apolipoprotein H.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792531

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Chimpanzee (98%).

Positive Control: human plasma, HepG2 sup.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2GPI; Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; Apo-H; Apolipoprotein H; apolipoprotein H (beta-2-glycoprotein I); B2GPI; beta(2)GPI; Beta-2-glycoprotein 1; Beta-2-glycoprotein I

Gene Aliases: APOH; B2G1; B2GP1; BG

UniProt ID: (Human) P02749

Entrez Gene ID: (Human) 350

Function(s)

Process(es)

glycoprotein binding protein binding phospholipid binding heparin binding lipid binding identical protein binding lipoprotein lipase activator activity

Apolipoprotein H Polyclonal Antibody

Product Details

PA5-85389

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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