Invitrogen

Arginase 1 Polyclonal Antibody

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Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Arginase I in the cytosol of paraffin-embedded Cal27 xenograft using an Arginase I polyclonal antibody (Product # PA5-29645) at a dilution of 1:500.

Arginase 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Arginase 1 Polyclonal Antibody (Product # PA5-29645) and a 35kDa band corresponding to Arginase-1 was observed. Tissue extracts (20 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:10,000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Band of interest was observed only in Liver tissue and not in Kidney.

Arginase 1 Antibody in Immunocytochemistry (ICC/IF) — Figure 4. Blocking CSF-1R inhibits proliferation of endometrial cancer cells. (A) Immunofluorescence staining of M1 macrophage (iNOS and CD86) and M2 macrophage (Arg-1 and CD206) in U937 cell lines, co-cultured with ECC-1/HEC-1A cell lines and treated with 100 U/ml M-CSF. (B) Cell counting kit-8 assay found that U937 cells could promote ECC-1 and HEC-1A cell proliferation. Additionally, the CSF-1R inhibitor PLX3397 (10 uM) inhibits proliferation of ECC-1 and HEC-1A cells in the co-culture system. (C) Immunofluorescence staining of Ki67 detecting EC cell proliferation. Data are presented as the mean +- standard deviation from 5 independent experiments; *P<0.05, **P<0.01 vs. Control. Scale bar: 50 um. Arg, arginase; CD, cluster of differentiation; CSF, colony-stimulating factor; CSF-1R, colony-stimulating factor 1 receptor; EC, endometrial cancer; iNOS, inducible nitric oxide synthase.

Arginase 1 Antibody in Immunohistochemistry (IHC) — Figure 6 MSC-Ex protects intestinal adherens junctions in an in vitro model. (A) Schematic representation of the in vitro co-culture system. Caco-2 cells were cultured on the upper transwell inserts in multiple well plates containing RAW 264.7 cells. To imitate inflammation in the gut, LPS was added to the basolateral compartment, and MSC-Ex was administered to the apical side. (B) Immunofluorescent staining for E-cadherin using FITC-conjugated antibodies. Continued epithelial barrier, indicated by E-cadherin staining, is disrupted in the absence of MSC-Ex. Magnification, 40x. (C) The transcripts of MCP-1 (M1 marker), CXCL9 (M1 marker), Arg-1(M2 marker), IL-10 (M2 marker), LIGHT (M2 marker), and CCL1 (M2 marker) in RAW 264.7 cells were examined by qRT-PCR analysis after 4 h of LPS (1 mug/mL) treatment and subsequent MSC-Ex (30 mug/mL) administration for 24 h. (D) The protein levels of IL-17 (top) and IL-10 (bottom) were measured by ELISA using culture supernatants from the basolateral side. Data are represented as the means +- s.d. ( n > 3 ). * P < 0.05, **P < 0.005, ***P < 0.001 .

Arginase 1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 4 MSC-Ex shifted the macrophage phenotype from M1 to M2 in the colon of mice with chronic colitis. The relative expressions of the macrophage phenotype-associated genes were evaluated by qRT-PCR: (A,B) MCP-1 and CXCL9 (M1 marker), (C,D) Arg-1 and IL-10 (M2 marker). (E) Representative photomicrographs of CD68 (macrophage marker), iNOS (M1 marker), and Arg-1 (M2 marker), immunohistochemically labeled in the colonic tissues of colitis mice. The photomicrographs were acquired at 100x magnification. (F) The protein levels of intracellular iNOS and Arg-1 were evaluated by western blotting. Data are represented as the means +- s.d. ( n >= 3 ). * P < 0.05, **P < 0.005, ***P < 0.001 .

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

1:1,000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 175 of Human Arginase I

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547120

Product Specific Information

Recommended positive controls: Mouse liver, rat liver.

Predicted reactivity: Mouse (85%), Rat (83%), Pig (90%), Rabbit (90%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176

Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846

Molecular Function: hydrolase