Invitrogen

Arginase 1 Polyclonal Antibody

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Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Arginase I in the cytosol of paraffin-embedded Cal27 xenograft using an Arginase I polyclonal antibody (Product # PA5-29645) at a dilution of 1:500.

Arginase 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Arginase 1 Polyclonal Antibody (Product # PA5-29645) and a 35kDa band corresponding to Arginase-1 was observed. Tissue extracts (20 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:10,000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Band of interest was observed only in Liver tissue and not in Kidney.

Arginase 1 Antibody — Figure 4 MSC-Ex shifted the macrophage phenotype from M1 to M2 in the colon of mice with chronic colitis. The relative expressions of the macrophage phenotype-associated genes were evaluated by qRT-PCR: (A,B) MCP-1 and CXCL9 (M1 marker), (C,D) Arg-1 and IL-10 (M2 marker). (E) Representative photomicrographs of CD68 (macrophage marker), iNOS (M1 marker), and Arg-1 (M2 marker), immunohistochemically labeled in the colonic tissues of colitis mice. The photomicrographs were acquired at 100x magnification. (F) The protein levels of intracellular iNOS and Arg-1 were evaluated by western blotting. Data are represented as the means +- s.d. ( n >= 3 ). * P < 0.05, **P < 0.005, ***P < 0.001 .

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:1000-1:10000

Immunocytochemistry (ICC)

Assay Dependent

View 1 publication 1 publication

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

View 1 publication 1 publication

Western Blot (WB)

1:5000-1:20,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 175 of Human Arginase I

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547120

Product Specific Information

Recommended positive controls: Mouse liver, rat liver.

Predicted reactivity: Mouse (85%), Rat (83%), Pig (90%), Rabbit (90%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176

Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846

Molecular Function: hydrolase