Invitrogen

Arginase 1 Polyclonal Antibody

Product Image Gallery

Arginase 1 Antibody — Figure 4 MSC-Ex shifted the macrophage phenotype from M1 to M2 in the colon of mice with chronic colitis. The relative expressions of the macrophage phenotype-associated genes were evaluated by qRT-PCR: (A,B) MCP-1 and CXCL9 (M1 marker), (C,D) Arg-1 and IL-10 (M2 marker). (E) Representative photomicrographs of CD68 (macrophage marker), iNOS (M1 marker), and Arg-1 (M2 marker), immunohistochemically labeled in the colonic tissues of colitis mice. The photomicrographs were acquired at 100x magnification. (F) The protein levels of intracellular iNOS and Arg-1 were evaluated by western blotting. Data are represented as the means +- s.d. ( n >= 3 ). * P < 0.05, **P < 0.005, ***P < 0.001 .

Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Arginase I in the cytosol of paraffin-embedded Cal27 xenograft using an Arginase I polyclonal antibody (Product # PA5-29645) at a dilution of 1:500.

Arginase 1 Antibody in Western Blot (WB) — Western blot analysis of Arginase I using 20 µg of mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Arginase I polyclonal antibody (Product # PA5-29645) at a dilution of 1:10,000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:5000-1:20,000

Published Applications

Immunohistochemistry (IHC)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 175 of Human Arginase I

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547120

Product Specific Information

PA5-29645 targets Arginase I in WB and IHC-P applications and shows reactivity with Human, Rat and mouse samples.

The PA5-29645 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 175 of Human Arginase I.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.