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|Tested species reactivity||Human|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 39 and 212 of Human Arp3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-30354 targets Arp3 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30354 immunogen is recombinant protein fragment corresponding to a region within amino acids 39 and 212 of Human Arp3.
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.
PA5-30354 was used in western blot to use 2D-DIGE and mass spectrometry to identify the protein changes in mammary epithelial cells
|Janjanam J,Singh S,Jena MK,Varshney N,Kola S,Kumar S,Kaushik JK,Grover S,Dang AK,Mukesh M,Prakash BS,Mohanty AK||PloS one (9:null)||2014|
actin-like protein 3; actin-related protein 3; ARP3; ARP3 actin-related protein 3 homolog