Invitrogen

Ataxin 3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

3 Published Figures

2 References

View all (12) Ataxin 3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

Ataxin 3 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of Ataxin 3 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 Polyclonal Antibody (Product # PA5 22369) diluted at 1:250. Blue: Hoechst 33342 staining.

Ataxin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Ataxin 3 in paraffin-embedded H661 xenograft using an Ataxin 3 polyclonal antibody (Product # PA5-22369) at a dilution of 1:500.

Ataxin 3 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extract (30 µg lysate) of HCT116 (Lane 1), HCT116 treated with thapsigargin (2uM, 6 hrs) (Lane 2), SH-SY5Y (Lane 3), and U-87MG (Lane 4). The blot was probed with Anti-Ataxin 3 Polyclonal Antibody (Product # PA5-22369, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 42 kDa band corresponding to Ataxin 3 was detected in cell lines tested.

Ataxin 3 Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of Ataxin 3 using with Ataxin 3 Polyclonal Antibody (Product # PA5-22369) shows an increase upon thapsigargin treatment. {TM}

Ataxin 3 Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in Ataxin3 knockout (KO) cell line compared to control cell line using Ataxin 3 Polyclonal Antibody (Product # PA5-22369). {KO}

Ataxin 3 Antibody — Published figure using Ataxin 3 polyclonal antibody (Product # PA5-22369)

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (Uniprot ID#P54252)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.98 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152091

Product Specific Information

Recommended positive controls: SK-N-SH, SK-N-AS, mouse brain, human Ataxin3-transfected 293T.

Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (85%), Pig (99%), Chicken (89%), Sheep (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ataxin 3 variant an; ataxin 3 variant ao; ataxin 3 variant at; ataxin 3 variant e; ataxin 3 variant h; ataxin 3 variant m; ataxin 3 variant r; ataxin 3 variant ref; ataxin 3 variant y; Ataxin-3; ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3); Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein

Gene Aliases: 2210008M02Rik; AI463012; AI647473; AT3; ataxin-3; ATX3; ATXN3; JOS; MJD; MJD1; SCA3

UniProt ID: (Human) P54252, (Mouse) Q9CVD2

Entrez Gene ID: (Human) 4287, (Mouse) 110616

Molecular Function: transcription factor