Proteintech

Aurora A Polyclonal Antibody

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Aurora A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10297-1-AP (Aurora A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Aurora A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10297-1-AP ( Aurora A antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Aurora A Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10297-1-AP ( Aurora A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

10297-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Aurora A Fusion Protein Ag0362 (108-337 aa encoded by BC002499)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: NNPEEELAS KQKNEESKKR QWALEDFEIG RPLGKGKFGN VYLAREKQSK FILALKVLFK AQLEKAGVEH QLRREVEIQS HLRHPNILRL YGYFHDATRV YLILEYAPLG TVYRELQKLS KFDEQRTATY ITELANALSY CHSKRVIHRD IKPENLLLGS AGELKIADFG WSVHAPSSRR TTLCGTLDYL PPEMIEGRMH DEKVDLWSLG VLCYEFLVGK PPFEANTYQE T (108-337 aa encoded by BC002499)

Target Information

Aurora A is a 46 kDa protein that plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Multiple transcript variants encoding the same protein have been found for this gene. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 EST 2022

Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in clear cell renal cell carcinoma and dual-targeting Aurora-A/SKP2 shows synthetic lethality.

Cell death & disease

Li P,Chen T,Kuang P,Liu F,Li Z,Liu F,Wang Y,Zhang W,Cai X

Wed Jul 13 00:00:00 EDT 2022

The phytochemical, corynoline, diminishes Aurora kinase B activity to induce mitotic defect and polyploidy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yan Z,Shi Q,Liu X,Li J,Ahire V,Zhang S,Zhang J,Yang D,Allen TD

Tue Mar 01 00:00:00 EST 2022

The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.

Frontiers in immunology

Wang Z,Mo Y,Tan Y,Wen Z,Dai Z,Zhang H,Zhang X,Feng S,Liang X,Song T,Cheng Q

Mon Feb 28 00:00:00 EST 2022

CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.

Nature communications

Zeng W,Yue L,Lam KSW,Zhang W,So WK,Tse EHY,Cheung TH

Thu Feb 17 00:00:00 EST 2022

Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma.

BMC cancer

Shi K,Zhang JZ,Yang L,Li NN,Yue Y,Du XH,Zhang XZ,Lu YC,Guo D

Wed Nov 10 00:00:00 EST 2021

Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.

Acta pharmaceutica Sinica. B

Zheng X,Li W,Xu H,Liu J,Ren L,Yang Y,Li S,Wang J,Ji T,Du G

Mon Nov 01 00:00:00 EDT 2021

Investigation on the clinical efficacy and mechanism of compound kushen injection in treating esophageal cancer based on multi-dimensional network meta-analysis and in vitro experiment.

Journal of ethnopharmacology

Zhou W,Huang Z,Wu C,Lu S,Fu C,Ye P,Tan Y,Wu Z,Fan X,Zhang J,Guo S,Jia S,Stalin A,Wang H,Zhang X,Wang M,Wu J

Thu Oct 28 00:00:00 EDT 2021

The Phytochemical Scoulerine Inhibits Aurora Kinase Activity to Induce Mitotic and Cytokinetic Defects.

Journal of natural products

Li J,Yan Z,Li H,Shi Q,Ahire V,Zhang S,Nimishetti N,Yang D,Allen TD,Zhang J

Fri Aug 27 00:00:00 EDT 2021

AURKA Increase the Chemosensitivity of Colon Cancer Cells to Oxaliplatin by Inhibiting the TP53-Mediated DNA Damage Response Genes.

BioMed research international

Shan B,Zhao R,Zhou J,Zhang M,Qi X,Wang T,Gong J,Wu Y,Zhu Y,Yang W,Zhang Y,Wang G,Li X

Thu Apr 29 00:00:00 EDT 2021

Avasimibe exerts anticancer effects on human glioblastoma cells via inducing cell apoptosis and cell cycle arrest.

Acta pharmacologica Sinica

Liu JY,Fu WQ,Zheng XJ,Li W,Ren LW,Wang JH,Yang C,Du GH

Fri Jan 01 00:00:00 EST 2021

Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.

European journal of medicinal chemistry

Fan C,Zhong T,Yang H,Yang Y,Wang D,Yang X,Xu Y,Fan Y

Sun Mar 15 00:00:00 EDT 2020

MiR-26a-5p Inhibits Cell Proliferation and Enhances Doxorubicin Sensitivity in HCC Cells via Targeting AURKA.

Technology in cancer research & treatment

Yuan YL,Yu H,Mu SM,Dong YD,Li Y

Tue Jan 01 00:00:00 EST 2019

Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.

Molecular pharmaceutics

Chen X,Liu C,Zhao R,Zhao P,Wu J,Zhou N,Ying M

Mon Nov 05 00:00:00 EST 2018

Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.

Molecular pharmaceutics

Chen X,Liu C,Zhao R,Zhao P,Wu J,Zhou N,Ying M

Mon Nov 05 00:00:00 EST 2018

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Journal of experimental & clinical cancer research : CR

Li W,Liu J,Fu W,Zheng X,Ren L,Liu S,Wang J,Ji T,Du G

Tue Jul 03 00:00:00 EDT 2018

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Journal of experimental & clinical cancer research : CR

Li W,Liu J,Fu W,Zheng X,Ren L,Liu S,Wang J,Ji T,Du G

Tue Jul 03 00:00:00 EDT 2018

Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.

Oncotarget

Liu Y,Hu H,Zhang C,Wang H,Zhang W,Wang Z,Li M,Zhang W,Zhou D,Jiang T

Tue Nov 10 00:00:00 EST 2015

Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.

Oncotarget

Liu Y,Hu H,Zhang C,Wang H,Zhang W,Wang Z,Li M,Zhang W,Zhou D,Jiang T

Tue Nov 10 00:00:00 EST 2015

Bioinformatics

Protein Aliases: ARK-1; AURAK; Aurora 2; Aurora A Kinase; Aurora kinase A; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; RP5-1167H4.6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1

Gene Aliases: AIK; AIRK1; ARK1; AURA; AURKA; AYK1; BTAK; IAK1; PPP1R47; STK15; STK6; STK7

UniProt ID: (Human) O14965

Entrez Gene ID: (Human) 6790

Molecular Function: non-receptor serine/threonine protein kinase