|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 343 and 403 of Aurora A (Uniprot ID#O14965)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-34700 targets Aurora A in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-34700 immunogen is synthetic peptide corresponding to a region within amino acids 343 and 403 of Aurora A (Uniprot ID#O14965).
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AIK; ARK-1; ARK1; AURA; AURAK; Aurora 2; aurora A; Aurora A Kinase; Aurora family kinase 1; Aurora Kinase A; aurora-A; aurora-related kinase 1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; BTAK; Ipl1- and aurora-related kinase 1; PPP1R47; protein phosphatase 1, regulatory subunit 47; ratAurA; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1; STK15; STK6; STK7
Gene Aliases: AIK; Airk; AIRK1; ARK-1; ARK1; AU019385; AURA; AURKA; Aurora-A; AW539821; AYK1; BTAK; IAK; IAK1; PPP1R47; STK15; STK6; STK7
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