Invitrogen

B3GAT1 Polyclonal Antibody

View all (8) B3GAT1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

B3GAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of B3GAT1 in paraffin-embedded mouse brain tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with B3GAT1 polyclonal antibody (Product # PA5-109336) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).

B3GAT1 Antibody in Western Blot (WB) — Western blot analysis of B3GAT1 in rat brain tissue lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with B3GAT1 polyclonal antibody (Product # PA5-109336), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5,000.

B3GAT1 Antibody in Flow Cytometry (Flow) — Flow cytometry of B3GAT1 in SH-SY5Y cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with B3GAT1 polyclonal antibody (Product # PA5-109336) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1,000 (30 min).

Product Details

PA5-109336

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Flow Cytometry (Flow)

1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within Human B3GAT1 aa 23-72

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

RRID

AB_2854747

Product Specific Information

Positive Control: Rat brain tissue, mouse brain tissue, SH-SY5Y.

Target Information

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Beta-1,3-glucuronyltransferase 1; CD57; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GLCATP; GlcUAT-P; GLCUATP; Glucuronosyltransferase P; LEU7 antigen; NK-1; NK1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Gene Aliases: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

UniProt ID: (Human) Q9P2W7

Entrez Gene ID: (Human) 27087

Molecular Function: glycosyltransferase

Function(s)

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process

Process(es)

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding