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Invitrogen
BAD Polyclonal Antibody
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FIGURE: 1 / 6
BAD Antibody (PA5-85624) in WB
Western Blot was performed using Anti-BAD Polyclonal Antibody (Product # PA5-85624) and a 18 kDa band corresponding to Bcl2-associated agonist of cell death was observed across cell lines. Whole cell extracts (40 µg lysate) of PC-3 (Lane 1), NIH:OVCAR-3 (Lane 2), HeLa (Lane 3), A-431 (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with ... View More
Product Details
PA5-85624
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Bad. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Rhesus Monkey (95%), Chimpanzee (100%).
Positive Control: 293T, A431, HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAD; BBC6; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; Bcl2-associated agonist of cell death; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; OTTMUSP00000017561; OTTMUSP00000022400; unnamed protein product
Gene Aliases: BAD; BBC2; BBC6; BCL2L8
UniProt ID: (Human) Q92934
Entrez Gene ID: (Human) 572
release of cytochrome c from mitochondria
glucose catabolic process
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of autophagy
positive regulation of mitochondrial membrane potential
cytokine-mediated signaling pathway
positive regulation of insulin secretion
positive regulation of insulin secretion involved in cellular response to glucose stimulus
glucose homeostasis
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
type B pancreatic cell proliferation
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of proteolysis
ADP metabolic process
ATP metabolic process
regulation of mitochondrial membrane permeability
pore complex assembly
epithelial cell proliferation
positive regulation of epithelial cell proliferation
cellular response to mechanical stimulus
cellular response to nicotine
cellular response to lipid
cellular response to hypoxia
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
positive regulation of type B pancreatic cell development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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