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Invitrogen
BAFF Polyclonal Antibody, Biotin, PeproTech®
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BAFF Antibody (500-P163GBT-1MG) in WB
Western Blot: To detect hBAFF by Western Blot analysis BAFF Polyclonal Antibody, Biotin (Product # 500-P163GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P163GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BAFF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929578
Product Specific Information
AA Sequence of recombinant protein: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBAFF.
Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P163GBT-1MG will be provided as 2 x 500 µg
Target Information
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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