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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide from near the carboxy terminus of human BANF1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (IHC)||20 ug/ml|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Despite its predicted molecular weight, BANF1 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human kidney tissue lysate.
PA5-20328 can be used with blocking peptide PEP-0448.
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BAF, BCRP1, D14S1460, NGPS; barrier-to-autointegration factor; breakpoint cluster region protein 1; LAP2-binding protein 1
BAF; BANF1; BCRG1; BCRP1; D14S1460; L2bp1; L2bp1/Baf; NGPS