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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, peptide sequence corresponding to residues 41-54 of Bcl-2 oncoprotein.|
|Storage buffer||tissue culture supernatant diluted in 6M urea|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:15-1:50|
|Western Blot (WB)||1:100-1:750|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26233 detects Bcl2 from human samples.
MA1-26233 has been successfully used in immunoprecipitation, Immunohistochemistry, and Western blot procedures. Positive control of Tonsil or human lung cell line suggested. This antibody works well with B5 or Bouin's fixed tissues. Formalin fixed tissues may give inconsistent results.
The MA1-26233 immunogen is recombinant, peptide sequence corresponding to residues 41-54 of Bcl-2 oncoprotein.
Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BCL2 and keratin 5 define the uterine-cervix-isthmus junction, a transition between endocervical and tubal-like epithelium.
MA1-26233 was used in immunohistochemistry to study the ability of BCL2 and keratin 5 to define the transition from the cervix to the lower uterine segment
|Hoogduin KJ,Hopman AN,Ramaekers FC,McCluggage WG,Smedts F||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (32:122)||2013|
Noxa up-regulation and Mcl-1 cleavage are associated to apoptosis induction by bortezomib in multiple myeloma.
MA1-26233 was used in western blot to study the mechanism by which bortezomib induces apoptosis in multiple myeloma
|Gomez-Bougie P,Wuillème-Toumi S,Ménoret E,Trichet V,Robillard N,Philippe M,Bataille R,Amiot M||Cancer research (67:5418)||2007|
B-cell CLL/lymphoma 2; Bcl-2; protein phosphatase 1, regulatory subunit 50
Bcl-2; BCL2; PPP1R50