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Invitrogen
BCLAF1 Polyclonal Antibody
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FIGURE: 1 / 2
BCLAF1 Antibody (PA5-85656) in ICC/IF
BCLAF1 Polyclonal Antibody detects BTF protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTF protein stained by BCLAF1 Polyclonal Antibody (Product # PA5-85656) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Product Details
PA5-85656
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human BTF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (94%), Rat (94%), Pig (98%), Bovine (98%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins. It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope. When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.BCLAF1 is found to express mostly in skeletal muscles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2-associated transcription factor 1; BCL2-associated transcription factor 1; BCLAF1 and THRAP3 family member 1; BP-1; Btf; Btf-s
Gene Aliases: BCLAF1; bK211L9.1; BTF; KIAA0164
UniProt ID: (Human) Q9NYF8
Entrez Gene ID: (Human) 9774
transcription, DNA-templated
apoptotic process
positive regulation of apoptotic process
regulation of DNA-templated transcription in response to stress
negative regulation of transcription, DNA-templated
positive regulation of DNA-templated transcription, initiation
positive regulation of response to DNA damage stimulus
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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