Invitrogen

BDKRB2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

View all (16) BDKRB2 antibodies

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BDKRB2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Cerebellum (Lane1), Rat Brain (Lane2), Mouse Brain (Lane3), Neuro-2a (Lane4), SH SY5Y (Lane5). The blots were probed with BDKRB2 Rabbit Polyclonal Antibody (Product # 720288, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 40 kDa band corresponding to BDKRB2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

BDKRB2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig 5 B2R-TP interactions in RASMC as revealed by co-IP followed by SDS PAGE. RASMC lysates were immunoprecipitated with anti-B2R followed by immunoblotting with anti-TP (upper panels) and anti-B2R (lower panels) antibodies, successively. E and L represent eluates and matching lysates of unstimulated RASMC (E1, L1), or RASMC that were stimulated with BK 10-11 M (E2, L2), IBOP 10-7 M (E3, L3), or [BK 10-11 M + IBOP 10-7 M] (E4, L4) for 10 min. EM and LM represent eluates and matching lysates of mock co-IP condition. (EB) represents eluates of a control co-IP condition, whereby Dynabeads-protein-A-anti-B2R were incubated with PBS instead of RASMC lysates. Images are representative of three qualitatively similar independent experiments. A denatured broad molecular weight protein ladder was loaded in parallel (upper and lower left-hand lanes).

BDKRB2 Antibody — Fig 5 B2R-TP interactions in RASMC as revealed by co-IP followed by SDS PAGE. RASMC lysates were immunoprecipitated with anti-B2R followed by immunoblotting with anti-TP (upper panels) and anti-B2R (lower panels) antibodies, successively. E and L represent eluates and matching lysates of unstimulated RASMC (E1, L1), or RASMC that were stimulated with BK 10-11 M (E2, L2), IBOP 10-7 M (E3, L3), or [BK 10-11 M + IBOP 10-7 M] (E4, L4) for 10 min. EM and LM represent eluates and matching lysates of mock co-IP condition. (EB) represents eluates of a control co-IP condition, whereby Dynabeads-protein-A-anti-B2R were incubated with PBS instead of RASMC lysates. Images are representative of three qualitatively similar independent experiments. A denatured broad molecular weight protein ladder was loaded in parallel (upper and lower left-hand lanes).

Product Details

720288

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to Human Bdkrb2 (aa 377-391)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2633240

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Rat, Mouse, Pig and Cat.

Target Information

Bradykinin B2 receptor (BDKRB2) expression has been reported in blood, brain, eye, kidney, liver, lung, prostate, skin, small intestine, synovium, uterus, and vessel. ESTs have been isolated from colon, connective tissue, heart, heart/melanocyte/uterus, and kidney libraries. It is associated with G-proteins that activate a phosphatidylinositol-calcium second messenger system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heteromerization fingerprints between bradykinin B2 and thromboxane TP receptors in native cells.

PloS one

Dagher OK,Jaffa MA,Habib A,Ziyadeh FN,Jaffa AA

720288 was used in Western Blotting to determine the effect of B2R-TP heterodimerisation on the coregulation of B2R and TP.

Wed Jan 22 00:00:00 EST 2020

Heteromerization fingerprints between bradykinin B2 and thromboxane TP receptors in native cells.

PloS one

Dagher OK,Jaffa MA,Habib A,Ziyadeh FN,Jaffa AA

720288 was used in Western Blotting to determine the effect of B2R-TP heterodimerisation on the coregulation of B2R and TP.

Wed Jan 22 00:00:00 EST 2020

Bioinformatics

Protein Aliases: B2 bradykinin receptor; B2R; BK-2 receptor; BK-2R; BK-2Rs; bradykinin receptor, beta 2; DKFZp686O088; kinin B2

Gene Aliases: B(2); B2; B2BKR; B2BRA; B2R; BDKRB2; BK-2; BK2; BK2R; BKR2; BRB2

UniProt ID: (Human) P30411, (Mouse) P32299, (Rat) P25023

Entrez Gene ID: (Human) 624, (Mouse) 12062, (Rat) 25245

Molecular Function: G-protein coupled receptor

acute inflammatory response to antigenic stimulus smooth muscle contraction inflammatory response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration blood circulation negative regulation of cell proliferation response to salt stress regulation of vasoconstriction negative regulation of peptidyl-serine phosphorylation maintenance of blood-brain barrier vasoconstriction vasodilation response to drug regulation of vascular permeability negative regulation of blood pressure arachidonic acid secretion cellular response to hypoxia negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator response to stress signal transduction negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress sensory perception of pain

Process(es)

protease binding phosphatidylinositol phospholipase C activity bradykinin receptor activity protein binding beta-2 adrenergic receptor binding type 1 angiotensin receptor binding protein heterodimerization activity signal transducer activity G-protein coupled receptor activity