Immunofluorescence analysis of BID (p15) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with BID (p15) Rabbit polyclonal Antibody (44433G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide derived from the human BID fragment (p15).|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sequential caspase-2 and caspase-8 activation is essential for saikosaponin a-induced apoptosis of human colon carcinoma cell lines.
44-433G was used in western blot to investigate the role of caspase activation in the apoptotic effect of saikosaponin in colon carcinoma cell lines
|Kim BM,Hong SH||Apoptosis : an international journal on programmed cell death (16:184)||2011|
|Not Applicable||Not Cited||
Desferrioxamine (DFX) induces apoptosis through the p38-caspase8-Bid-Bax pathway in PHA-stimulated human lymphocytes.
44-433G was used in western blot to investigate the effect of desferrioxamine on apoptosis and its mechanism
|Kim BM,Chung HW||Toxicology and applied pharmacology (228:24)||2008|
|Not Applicable||Not Cited||
Hypoxia/reoxygenation induces apoptosis through a ROS-mediated caspase-8/Bid/Bax pathway in human lymphocytes.
44-433G was used in western blot to elucidate the mechanism of hypoxia/reoxygenation-induced apoptosis in human cultured lymphocytes
|Kim BM,Chung HW||Biochemical and biophysical research communications (363:745)||2007|
TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window.
44-433G was used in western blot to examine the hepatotoxicity of TRAIL/bortezomib cotreatment
|Koschny R,Ganten TM,Sykora J,Haas TL,Sprick MR,Kolb A,Stremmel W,Walczak H||Hepatology (Baltimore, Md.) (45:649)||2007|
|Mouse||Not Cited||Early growth response gene 1-mediated apoptosis is essential for transforming growth factor beta1-induced pulmonary fibrosis.||Lee CG,Cho SJ,Kang MJ,Chapoval SP,Lee PJ,Noble PW,Yehualaeshet T,Lu B,Flavell RA,Milbrandt J,Homer RJ,Elias JA||The Journal of experimental medicine (200:377)||2004|
|Adenoviral Bid overexpression induces caspase-dependent cleavage of truncated Bid and p53-independent apoptosis in human non-small cell lung cancers.||Fukazawa T,Walter B,Owen-Schaub LB||The Journal of biological chemistry (278:25428)||2003|