Proteintech

BMI1 Polyclonal Antibody

BMI1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

BMI1 Antibody (10832-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

BMI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMI1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10832-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

ChIP assay (ChIP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BMI1 Fusion Protein Ag1286 (1-326 aa encoded by BC011652)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody 10832-1-AP detected proteins of 45-48 kDa, which may include phosphorylated isoforms of the protein.

Immunogen sequence: MHRTTRIKI TELNPHLMCV LCGGYFIDAT TIIECLHSFC KTCIVRYLET SKYCPICDVQ VHKTRPLLNI RSDKTLQDIV YKLVPGLFKN EMKRRRDFYA AHPSADAANG SNEDRGEVAD EDKRIITDDE IISLSIEFFD QNRLDRKVNK DKEKSKEEVN DKRYLRCPAA MTVMHLRKFL RSKMDIPNTF QIDVMYEEEP LKDYYTLMDI AYIYTWRRNG PLPLKYRVRP TCKRMKISHQ RDGLTNAGEL ESDSGSDKAN SPAGGIPSTS SCLPSPSTPV QSPHPQFPHI SSTMNGTSNS PSGNHQSSFA NRPRKSSVNG SSATSSG (1-326 aa encoded by BC011652)

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

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Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

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Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

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Wed Feb 02 00:00:00 UTC 2011

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

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Wed Feb 02 00:00:00 UTC 2011

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

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Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.