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BMI1 Polyclonal Antibody

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BMI1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

BMI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 40x lens).

BMI1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

BMI1 Antibody in Immunoprecipitation (IP) — IP result of anti-BMI1 (IP: 10832-1-AP, 4 µg; Detection: 10832-1-AP 1:500) with U-937 cells lysate 4000 µg.

Product Details

10832-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 9 publications 9 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BMI1 Fusion Protein Ag1286 (1-326 aa encoded by BC011652) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

The antibody 10832-1-AP detected proteins of 45-48 kDa, which may include phosphorylated isoforms of the protein.

Immunogen sequence: MHRTTRIKI TELNPHLMCV LCGGYFIDAT TIIECLHSFC KTCIVRYLET SKYCPICDVQ VHKTRPLLNI RSDKTLQDIV YKLVPGLFKN EMKRRRDFYA AHPSADAANG SNEDRGEVAD EDKRIITDDE IISLSIEFFD QNRLDRKVNK DKEKSKEEVN DKRYLRCPAA MTVMHLRKFL RSKMDIPNTF QIDVMYEEEP LKDYYTLMDI AYIYTWRRNG PLPLKYRVRP TCKRMKISHQ RDGLTNAGEL ESDSGSDKAN SPAGGIPSTS SCLPSPSTPV QSPHPQFPHI SSTMNGTSNS PSGNHQSSFA NRPRKSSVNG SSATSSG (1-326 aa encoded by BC011652)

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease.

Cell cycle (Georgetown, Tex.)

Su Y,Wen Z,Shen Q,Zhang H,Peng L,Chen G,Zhu Z,Du C,Xie H,Li H,Xia Y,Tang W

Mon Sep 09 00:00:00 EDT 2019

Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.

International journal of medical sciences

Zang J,Hui L,Yang N,Yang B,Jiang X

Fri Aug 31 00:00:00 EDT 2018

Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.

International journal of medical sciences

Zang J,Hui L,Yang N,Yang B,Jiang X

Fri Aug 31 00:00:00 EDT 2018

SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.

International journal of cancer

Xie SL,Fan S,Zhang SY,Chen WX,Li QX,Pan GK,Zhang HQ,Wang WW,Weng B,Zhang Z,Li JS,Lin ZY

Thu Mar 15 00:00:00 EDT 2018

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Oncotarget

Wei X,Ye J,Shang Y,Chen H,Liu S,Liu L,Wang R

Tue Dec 12 00:00:00 EST 2017

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

The EMBO journal

Wang X,Cao W,Zhang J,Yan M,Xu Q,Wu X,Wan L,Zhang Z,Zhang C,Qin X,Xiao M,Ye D,Liu Y,Han Z,Wang S,Mao L,Wei W,Chen W

Tue May 02 00:00:00 EDT 2017

Preclinical activity of the novel B-cell-specific Moloney murine leukemia virus integration site 1 inhibitor PTC-209 in acute myeloid leukemia: Implications for leukemia therapy.

Cancer science

Nishida Y,Maeda A,Chachad D,Ishizawa J,Qiu YH,Kornblau SM,Kimura S,Andreeff M,Kojima K

Tue Dec 01 00:00:00 EST 2015

Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

The FEBS journal

Wang HB,Liu GH,Zhang H,Xing S,Hu LJ,Zhao WF,Xie B,Li MZ,Zeng BH,Li Y,Zeng MS

Sat Jun 01 00:00:00 EDT 2013

Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

The FEBS journal

Wang HB,Liu GH,Zhang H,Xing S,Hu LJ,Zhao WF,Xie B,Li MZ,Zeng BH,Li Y,Zeng MS

Sat Jun 01 00:00:00 EDT 2013

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

PloS one

Wu Z,Min L,Chen D,Hao D,Duan Y,Qiu G,Wang Y

Wed Feb 02 00:00:00 EST 2011

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

PloS one

Wu Z,Min L,Chen D,Hao D,Duan Y,Qiu G,Wang Y

Wed Feb 02 00:00:00 EST 2011

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kim MO,Chawla P,Overland RP,Xia E,Sadri-Vakili G,Cha JH

Wed Apr 09 00:00:00 EDT 2008

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kim MO,Chawla P,Overland RP,Xia E,Sadri-Vakili G,Cha JH

Wed Apr 09 00:00:00 EDT 2008

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kim MO,Chawla P,Overland RP,Xia E,Sadri-Vakili G,Cha JH

Wed Apr 09 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

Gene Aliases: AW546694; Bmi-1; BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51

UniProt ID: (Human) P35226, (Mouse) P25916

Entrez Gene ID: (Human) 648, (Mouse) 12151

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated segment specification regulation of gene expression covalent chromatin modification protein sumoylation hemopoiesis negative regulation of gene expression, epigenetic positive regulation of fibroblast proliferation positive regulation of ubiquitin-protein transferase activity skeletal system development in utero embryonic development DNA methylation regulation of transcription, DNA-templated humoral immune response brain development cellular process chromatin modification histone acetylation histone ubiquitination rostrocaudal neural tube patterning positive regulation of B cell proliferation positive regulation of immature T cell proliferation in thymus somatic stem cell division embryonic skeletal system morphogenesis embryonic skeletal system development regulation of catalytic activity hematopoietic stem cell homeostasis negative regulation of apoptotic signaling pathway

Process(es)

protein binding zinc ion binding RING-like zinc finger domain binding promoter-specific chromatin binding chromatin binding sequence-specific DNA binding metal ion binding ubiquitin-protein transferase activator activity

BMI1 Polyclonal Antibody

Product Details

10832-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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