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Invitrogen
CD11a (LFA-1alpha) Monoclonal Antibody (R7.1), eBioscience™
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Product Details
BMS102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cat,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
R7.1
Immunogen
Rec. Human CD11a (LFA-1alpha)
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This antibody specifically recognises CD11a, otherwise known as integrin alpha L chain. Clone R7.1 recognises an epitope within the I-domain of CD11a and can be used in neutralization studies.
BMS102 is also suitable as primary antibody in staining for FACS analysis, BMS102FI can be used for direct staining of cells, BMS102BT can be used in a 2-step procedure using the biotin-(strept)avidin system.
CD11a is a 180kDa glycoprotein and member of the integrin family of cell adhesion molecules, which is expressed by all human leukocytes.CD11a combines with the CD18 beta chain (ITGB2) to form lymphocyte function-associated antigen-1 (LFA-1), and binds to its major ligands CD50 (ICAM3), CD54 (ICAM1) and CD102 (ICAM2), acting as a key mediator of cell-cell adhesion processes in normal immune and inflammatory responses.
Applications Tested: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry.
Purity: >95%.
Target Information
CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; Integrin alpha-L; integrin alphaL; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide
Gene Aliases: CD11A; ITGAL; LFA-1; LFA1A
UniProt ID: (Human) P20701
Entrez Gene ID: (Human) 3683
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
phagocytosis
movement of cell or subcellular component
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
signal transduction
integrin-mediated signaling pathway
extracellular matrix organization
receptor clustering
regulation of immune response
leukocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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