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CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™
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FIGURE: 1 / 7
CD54 (ICAM-1) Antibody (BMS108FI) in Flow
Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells (blue histogram) or Raji Cas9 control cells (pink histogram) with10 µl CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™ (Product # BMS108FI). Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone RR1/1 but not in the contr... View More
Product Details
BMS108FI
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RR1/1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Recognizes the D1 domain of human CD54.
Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors.
ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches.
ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.
Applications Tested: Flow Cytometry.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; Human rhinovirus receptor; ICAM-1; ICAM-1 soluble variant; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1 precursor; Ly 47; Major group rhinovirus receptor; major group rhinovirus receptor precusor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells; unnamed protein product
Gene Aliases: BB2; CD54; ICAM1; P3.58
UniProt ID: (Human) P05362
Entrez Gene ID: (Human) 3383
regulation of leukocyte mediated cytotoxicity
immune effector process
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
T cell antigen processing and presentation
positive regulation of cellular extravasation
immune response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
membrane to membrane docking
cell adhesion mediated by integrin
adhesion of symbiont to host
viral entry into host cell
receptor-mediated virion attachment to host cell
leukocyte migration
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to glucose stimulus
T cell extravasation
cell-cell adhesion
regulation of ruffle assembly
cellular response to oxygen-containing compound
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
cellular response to beta-amyloid
cellular response to leukemia inhibitory factor
negative regulation of endothelial cell apoptotic process
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