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CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™
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FIGURE: 1 / 7
CD54 (ICAM-1) Antibody (BMS108FI) in Flow
Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells (blue histogram) or Raji Cas9 control cells (pink histogram) with10 µl CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™ (Product # BMS108FI). Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone RR1/1 but not in the contr... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
BMS108FI
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RR1/1
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Recognizes the D1 domain of human CD54.
Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors.
ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches.
ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.
Applications Tested: Flow Cytometry.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; Human rhinovirus receptor; ICAM-1; ICAM-1 soluble variant; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1 precursor; Ly 47; major group rhinovirus receptor; major group rhinovirus receptor precusor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells; unnamed protein product
Gene Aliases: BB2; CD54; P3.58
UniProt ID: (Human) P05362
Entrez Gene ID: (Human) 3383
regulation of leukocyte mediated cytotoxicity
immune effector process
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of cellular extravasation
immune response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
signal transduction
membrane to membrane docking
cell-cell adhesion mediated by integrin
adhesion of symbiont to host
receptor-mediated virion attachment to host cell
leukocyte migration
establishment of endothelial barrier
leukocyte adhesion to vascular endothelial cell
positive regulation of ERK1 and ERK2 cascade
cell-cell adhesion
cellular response to oxygen-containing compound
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
cellular response to beta-amyloid
negative regulation of endothelial cell apoptotic process
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