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Invitrogen
IL-1 beta Monoclonal Antibody (B-A15), FITC, eBioscience™
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Product Details
BMS127FI
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B-A15
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: BMS127 recognizes natural and recombinant human IL1 beta.
IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils. It belongs to the IL-1 family cluster. Associated activities with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption.
Applications Tested: Flow Cytometry (Intracellular Staining).
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1 precursor polypeptide; interleukin 1beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta; unnamed protein product
Gene Aliases: IL-1; IL1-BETA; IL1beta; IL1F2
UniProt ID: (Human) P01584
Entrez Gene ID: (Human) 3553
positive regulation of cytokine production
microglial cell activation involved in immune response
positive regulation of T cell mediated immunity
apoptotic process
inflammatory response
immune response
signal transduction
cell-cell signaling
embryo implantation
positive regulation of cell proliferation
negative regulation of cell proliferation
vascular endothelial growth factor production
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
positive regulation of platelet-derived growth factor receptor signaling pathway
positive regulation of epithelial to mesenchymal transition
positive regulation of macrophage derived foam cell differentiation
negative regulation of glucose transport
smooth muscle adaptation
cytokine-mediated signaling pathway
hyaluronan biosynthetic process
monocyte differentiation
positive regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of prostaglandin biosynthetic process
positive regulation of fever generation
positive regulation of prostaglandin secretion
response to lipopolysaccharide
negative regulation of interleukin-1 beta production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-2 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of immature T cell proliferation in thymus
positive regulation of heterotypic cell-cell adhesion
positive regulation of T cell proliferation
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAPK cascade
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
negative regulation of lipid metabolic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
positive regulation of complement activation
positive regulation of JNK cascade
negative regulation of insulin receptor signaling pathway
positive regulation of protein export from nucleus
regulation of defense response to virus by host
negative regulation of inflammatory response
positive regulation of inflammatory response
regulation of neurogenesis
negative regulation of neurogenesis
regulation of insulin secretion
negative regulation of synaptic transmission
defense response to Gram-positive bacterium
negative regulation of lipid catabolic process
positive regulation of lipid catabolic process
regulation of nitric-oxide synthase activity
positive regulation of membrane protein ectodomain proteolysis
positive regulation of protein kinase B signaling
positive regulation of glial cell proliferation
positive regulation of cell adhesion molecule production
negative regulation of adiponectin secretion
regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
interleukin-1-mediated signaling pathway
response to interleukin-1
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to xenobiotic stimulus
positive regulation of monocyte chemotactic protein-1 production
cellular response to interleukin-17
positive regulation of neuroinflammatory response
positive regulation of p38MAPK cascade
positive regulation of NIK/NF-kappaB signaling
positive regulation of RNA biosynthetic process
regulation of establishment of endothelial barrier
regulation of secretion by cell
negative regulation of gap junction assembly
positive regulation of occluding junction disassembly
positive regulation of T-helper 1 cell cytokine production
negative regulation of interleukin-1-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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