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Invitrogen
CD26 Monoclonal Antibody (M-A261), Biotin, eBioscience™
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FIGURE: 1 / 3
CD26 Antibody (BMS143BT) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC and CD26 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD26 clone M-A261 showes high levels of CD26 in a subset of lymphocytes (pink) and low level of CD26 in monocytes (orange) and granulocytes (blue).
Product Details
BMS143BT
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
M-A261
Conjugate
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
2-8°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: CD26 is a 110 kD type II membrane protein encoded by the DPP4 gene. It is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It also plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45 and ADA.
Immunohistochemistry:
The antibody can be used to stain cryostat sections. Recommended for BMS143 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections.
Flow cytometry:
BMS143 is also suitable as primary antibody in staining for FACS analysis, BMS143FI can be used for direct staining of cells, BMS143BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended dilution is 1:20 as determined on A704 cells.
Immunoprecipitation:
BMS143 is also suitable for immunoprecipitation. Optimal dilutions should be determined by the individual laboratory for each application.
Applications Tested: Flow Cytometry.
Purity: >95%.
Target Information
CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADABP; ADCP-2; Adenosine deaminase complexing protein 2; CD26; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP IV; DPP-IV; Gly-Pro naphthylamidase; Post-proline dipeptidyl aminopeptidase IV; T-cell activation antigen CD26; TP103; Xaa-Pro-dipeptidylaminopeptidase
Gene Aliases: ADABP; ADCP2; CD26; DPP4; DPPIV; TP103
UniProt ID: (Human) P27487
Entrez Gene ID: (Human) 1803
virus receptor activity
protease binding
aminopeptidase activity
serine-type endopeptidase activity
receptor binding
protein binding
peptidase activity
serine-type peptidase activity
dipeptidyl-peptidase activity
hydrolase activity
identical protein binding
protein homodimerization activity
chemorepellent activity
behavioral fear response
response to hypoxia
proteolysis
cell adhesion
positive regulation of cell proliferation
negative regulation of extracellular matrix disassembly
peptide hormone processing
receptor-mediated endocytosis of virus by host cell
T cell costimulation
regulation of cell-cell adhesion mediated by integrin
locomotory exploration behavior
psychomotor behavior
T cell activation
endothelial cell migration
viral entry into host cell
receptor-mediated virion attachment to host cell
negative chemotaxis
membrane fusion
negative regulation of neutrophil chemotaxis
glucagon processing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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