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Invitrogen
CD44var (v3) Monoclonal Antibody (VFF-327v3), eBioscience™
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FIGURE: 1 / 4
CD44var (v3) Antibody (BMS144) in ICC/IF
Life science alliance 2019 -
Figure 4. Knockdown of GRP78 or CD44 alters cell morphology, reduces cell attachment, and impedes cell spreading in MCF7-LR cells. (A) Left: Western blot analysis of WCLs prepared from MCF7-LR cells transfected with control siRNA (sictrl) or siRNAs targeting GRP78 coding sequence, si78(1), or 3' untranslated region (3'-UTR), si78(2), using antibodies against GRP78 (MAb159) or HSP70 (C92F3A-5). GAPDH served... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
BMS144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
VFF-327v3
Immunogen
Rec. Human CD44var (v3-v10)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: Specifically recognizes an epitope encoded by exon v3 on the variant portion of human CD44.
Standard CD44 (CD44s) lacks the entire variable region and is found mainly in cells of lymphohematopoietic origin. The multiple isoforms (designated CD44v; v = variant) contain alternatively spliced exons inserted within the extracellular domain. These isoforms are known as CD44v1, CD44v2, CD44v3, CD44v4, CD44v5, CD44v6, CD44v7, CD44v8, CD44v9, CD44v10, CD44v11.
Applications Tested: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blotting.
Target Information
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AL133330.1; CD44 molecule (Indian blood group); CD44 splice variant; CD44 var; CD44 variant; CD44R1; CD44v3; CDw44 antigen precursor; cell surface glycoprotein CD44; cell-cell and cell-matrix adhesion interaction glycoprotein isoform; cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; ECMRIII; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; H-CAM v3; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; Hermes antigen gp90 homing receptor precursor; homing cell adhesion molecule; homing function and Indian blood group system; homing receptor; HUTCHI; hyaluronan-binding protein; hyaluronate receptor; In(Lu) related-p80; Indian blood group antigen; MGC10468; MUTCH-I; PGP I; phagocyte glycoprotein 1; phagocytic glycoprotein 1; soluble CD44; unnamed protein product
Gene Aliases: CDW44; CSPG8; ECM-III; ECMR-III; H-CAM; HCELL; Hermes-1; HUTCH-1; HUTCH-I; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
UniProt ID: (Human) P16070
Entrez Gene ID: (Human) 960
endocytosis
inflammatory response
cell adhesion
cell-matrix adhesion
anatomical structure morphogenesis
cell migration
cytokine-mediated signaling pathway
hyaluronan catabolic process
positive regulation of heterotypic cell-cell adhesion
T cell activation
regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
wound healing, spreading of cells
cellular response to fibroblast growth factor stimulus
system development
NK T cell activation
cartilage development
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
cell-cell adhesion
positive regulation of monocyte aggregation
carbohydrate derivative transport
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of lamellipodium morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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